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公开(公告)号：WO2014035712A1
公开(公告)日：2014-03-06
申请号：PCT/US2013/055639
申请日：2013-08-20
申请人： PIERCE BIOTECHNOLOGY, INC. , DYOMICS GMBH , HERMANSON, Greg , CZERNEY, Peter, T. , DESAI, Surbhi , WENZEL, Matthias, S. , LEHMANN, Frank, G. , NLEND, Marie, Christine
发明人： HERMANSON, Greg , CZERNEY, Peter, T. , DESAI, Surbhi , WENZEL, Matthias, S. , LEHMANN, Frank, G. , NLEND, Marie, Christine
IPC分类号： C07D405/06 , C07D405/14 , A61K49/10
CPC分类号： C07D491/147 , A61K49/0021 , A61K49/0032 , A61K49/0054 , A61K49/0058 , C07D405/06 , C07D405/14 , C07D491/052 , C07D491/16 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/086 , C09B69/00 , G01N33/582
摘要： Compounds used as labels with properties comparable to known fluorescent compounds. The compounds are conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non- limiting examples of each are provided.
摘要翻译： 用作具有与已知荧光化合物相当的性质的标签的化合物。 这些化合物与蛋白质和核酸结合，用于生物成像和分析。 提供化合物的合成，共轭化合物的形成和用途，以及各自的具体非限制性实例。
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公开(公告)号：WO02073157A3
公开(公告)日：2003-03-20
申请号：PCT/US0206876
申请日：2002-03-07
发明人： DESAI SURBHI
IPC分类号： G01N33/58 , C12Q1/28 , C12Q1/42 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N27/447 , G01N33/561 , G01N33/53 , G01N33/542
CPC分类号： C12Q1/6813 , G01N27/44717 , G01N27/44726 , G01N33/561 , C12Q2565/125
摘要： An improved method for detecting a target biomolecule directly in a polyacrylamide gel in which it has been separated from other substances. The improvement resides in, prior to binding the target biomolecule to a probe and while the target biomolecule remains in the gel, (1) immersing the gel in a water miscible, aqueous extracting medium to shrink the gel by at least about ten percent and then (2) washing the gel with water to restore the gel to substantially its original size.
摘要翻译： 一种用于直接在聚丙烯酰胺凝胶中检测目标生物分子的改进方法，其中已经与其它物质分离。 改进在于将目标生物分子结合到探针之前，并且当目标生物分子保留在凝胶中时，（1）将凝胶浸入与水混溶的水提取介质中，以将凝胶收缩至少约百分之十，然后 （2）用水洗涤凝胶以将凝胶恢复至其原始尺寸。
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公开(公告)号：WO2012088007A1
公开(公告)日：2012-06-28
申请号：PCT/US2011/065975
申请日：2011-12-20
申请人： PIERCE BIOTECHNOLOGY, INC. , DYOMICS GMBH, , HERMANSON, Greg , CZERNEY, Peter, T. , DESAI, Surbhi , WENZEL, Matthias, S. , DWORECKI, Boguslawa , LEHMANN, Frank, G.
发明人： HERMANSON, Greg , CZERNEY, Peter, T. , DESAI, Surbhi , WENZEL, Matthias, S. , DWORECKI, Boguslawa , LEHMANN, Frank, G.
IPC分类号： C07D209/10 , C07D403/06
CPC分类号： C07D403/06 , C07D209/10
摘要： Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non- limiting examples of each are provided.
摘要翻译： 用作具有与已知荧光化合物相当的特性的标签的化合物。 这些化合物可以与蛋白质和核酸结合，用于生物成像和分析。 提供化合物的合成，共轭化合物的形成和用途，以及各自的具体非限制性实例。
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公开(公告)号：WO2009055387A8
公开(公告)日：2009-04-30
申请号：PCT/US2008/080640
申请日：2008-10-21
摘要： Polymerized conjugates of at least two proteins that have increased sensitivity for detecting proteins in biological assays such as gels and blots, and methods of preparing the conjugates, and a protein assay detection kit using the conjugates. In one embodiment, proteins are polymerized by reacting a carbohydrate group on the protein that has been oxidized with an amine group.
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公开(公告)号：WO2002073157A2
公开(公告)日：2002-09-19
申请号：PCT/US2002/006876
申请日：2002-03-07
发明人： DESAI, Surbhi
IPC分类号： G01N
CPC分类号： C12Q1/6813 , G01N27/44717 , G01N27/44726 , G01N33/561 , C12Q2565/125
摘要： An improved method for detecting a target biomolecule directly in a polyacrylamide gel in which it has been separated from other substances. The improvement resides in, prior to binding the target biomolecule to a probe and while the target biomolecule remains in the gel, (1) immersing the gel in a water miscible, aqueous extracting medium to shrink the gel by at least about ten percent and then (2) washing the gel with water to restore the gel to substantially its original size.
摘要翻译： 一种用于直接在聚丙烯酰胺凝胶中检测目标生物分子的改进方法，其中已经与其它物质分离。 改进在于将目标生物分子结合到探针之前，并且当目标生物分子保留在凝胶中时，（1）将凝胶浸入与水混溶的水提取介质中，以将凝胶收缩至少约百分之十，然后 （2）用水洗涤凝胶以将凝胶恢复至其原始尺寸。
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公开(公告)号：WO2013130761A1
公开(公告)日：2013-09-06
申请号：PCT/US2013/028252
申请日：2013-02-28
申请人： PIERCE BIOTECHNOLOGY, INC. , DYOMICS, Gmbh , HERMANSON, Greg , CZERNEY, Peter, T. , DESAI, Surbhi , WENZEL, Matthias, S. , DWORECKI, Boguslawa, R , LEHMANN, Frank, G. , NLEND, Marie, Christine
发明人： HERMANSON, Greg , CZERNEY, Peter, T. , DESAI, Surbhi , WENZEL, Matthias, S. , DWORECKI, Boguslawa, R , LEHMANN, Frank, G. , NLEND, Marie, Christine
IPC分类号： C07D403/06 , C07D403/08 , C09B23/00 , G01N33/58
CPC分类号： C07D401/14 , A61K47/6871 , A61K49/04 , A61K49/12 , A61K49/143 , A61K49/16 , A61K49/221 , C07D209/18 , C07D403/06 , C07D403/08 , C07K16/40 , C07K2317/76 , C09B23/06 , G01N33/582
摘要： Indole derivatives used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific nonlimiting examples of each are provided.
摘要翻译： 用作具有与已知荧光化合物相当的性质的标记的吲哚衍生物。 这些化合物可以与蛋白质和核酸结合，用于生物成像和分析。 提供了化合物的合成，共轭化合物的形成和使用以及各自的具体非限制性实例。
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公开(公告)号：WO2009055387A1
公开(公告)日：2009-04-30
申请号：PCT/US2008080640
申请日：2008-10-21
CPC分类号： C07K19/00 , C12N9/0004 , G01N33/535 , G01N33/542 , G01N33/581
摘要： Polymerized conjugates of at least two proteins that have increased sensitivity for detecting proteins in biological assays such as gels and blots, and methods of preparing the conjugates, and a protein assay detection kit using the conjugates. In one embodiment, proteins are polymerized by reacting a carbohydrate group on the protein that has been oxidized with an amine group.
摘要翻译： 至少两种在生物测定如凝胶和印迹中检测蛋白质的敏感性增加的蛋白质的聚合缀合物，以及制备缀合物的方法，以及使用该共轭物的蛋白质测定检测试剂盒。 在一个实施方案中，通过使已被氧化的蛋白质上的碳水化合物基团与胺基团反应来使蛋白质聚合。
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