公开(公告)号：WO9419472A3

公开(公告)日：1994-11-24

申请号：PCT/GB9400373

申请日：1994-02-25

申请人： HEALTH LAB SERVICE BOARD ,  MINTON NIGEL PETER ,  FAULKNER JAMES D B

发明人： MINTON NIGEL PETER ,  FAULKNER JAMES DUNCAN BRUCE

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N1/21 ,  C12N9/88 ,  C12N15/66 ,  C12N15/70 ,  C12N15/81 ,  C12P21/02 ,  C12R1/19 ,  C12R1/21 ,  C12N15/67

CPC分类号： C12N15/70 ,  C12N9/88 ,  C12N15/66 ,  C12N15/81

摘要： PCT No. PCT/GB94/00373 Sec. 371 Date Sep. 8, 1995 Sec. 102(e) Date Sep. 8, 1995 PCT Filed Feb. 25, 1994 PCT Pub. No. WO94/19472 PCT Pub. Date Sep. 1, 1994A novel expression system is provided comprising a DNA sequence containing transcriptional and translational signals that promote the over production of recombinant proteins both in bacterial hosts (e.g., Escherichia coli) and yeasts (e.g., Saccharomyces cerevisiae). The design of the expression system lends itself to a unique strategy which allows heterologous genes to be directly cloned at a position relative to the transcription/translation signals which is optimal for expression. Particularly provided are expression cassettes comprising a sequence of the invention combined with a purpose built series of plasmids wherein the utility and efficiency of the resultant expression vectors can be demonstrated to over produce protein, particularly phenylalanine ammonia lyase (herein abbreviated to PAL), in E. coli and S. cerevisiae to levels hitherto unattainable.