公开(公告)号：WO9847922A3

公开(公告)日：1999-01-21

申请号：PCT/US9808037

申请日：1998-04-21

Applicant: GLYCOZYME INC

Inventor： MURRAY ALLEN K

IPC: C07K16/22 ,  C12P21/08 ,  G01N33/74 ,  C07K16/18 ,  A61K39/00 ,  G01N33/68

CPC classification number: G01N33/746 ,  C07K16/22

Abstract: Polyclonal antibodies can be produced that reacts with recombinant EPO and its degradation products but not with native EPO. This antibody precipitation can be used to identify those glycopeptides that are uniquely reactive. These glycopeptides can be produced on preparative scale and used in the production of monoclonal antibodies which are screened against the original EPO and glycopeptides to select antibodies reactive to the specific glycopeptides an recombinant EPO but not to native human EPO. The monoclonal antibodies so selected are incorporated in a conventional ELISA and used to monitor urine and other bodily samples taken from athletes, either human or animal, and patients for presence and level of recombinant peptides or proteins. Alternatively, the polyclonal antibody can be used directly to produce ELISA test.

Abstract translation: 可以产生与重组EPO及其降解产物反应但不与天然EPO反应的多克隆抗体。 这种抗体沉淀可用于鉴定唯一反应性的那些糖肽。 这些糖肽可以按制备规模生产，并用于生产单克隆抗体，其针对原始EPO和糖肽进行筛选以选择对特异性糖肽具有反应性的重组EPO而不是天然人EPO。 所选择的单克隆抗体掺入常规ELISA中，并用于监测从人或动物运动员获得的尿液和其他身体样品，以及患者存在和水平的重组肽或蛋白质。 或者，多克隆抗体可以直接用于产生ELISA测试。

公开(公告)号：WO2009047583A2

公开(公告)日：2009-04-16

申请号：PCT/IB2007/004690

申请日：2007-01-04

Applicant: GLYCOZYME, INC. ,  MURRAY, Allen, K.

Inventor： MURRAY, Allen, K.

CPC classification number: G01N33/362 ,  G01N30/96 ,  G01N2030/8809 ,  Y10T436/143333 ,  B01D15/363

Abstract: This patent application is for the use of acetic nitric reagent (80% acetic acid, 1.8 N nitric acid) for the extraction of oligosaccharides and polysaccharides from carbohydrate containing materials. The material is extracted with the acetic nitric reagent in a boiling water bath for various periods of time, usually 30 minutes. The material is then centrifuged and the clear, yellowish, supernatant is then taken to dryness in a Speed Vac under reduced pressure. The dry residue is then taken up in water and centrifuged to remove particulates. The resulting supernatant is then analyzed by high pH anion exchange chromatography with integrated amperometric detection. The resulting chromatogram or the integrated areas under the peaks are then characteristic for that particular source of material. This method is useful for analysis of cotton fibers, wood, paper, textiles or any cellulosic material. This method is an additional method utilized to characterize these materials as described in US Patent No. 06562626B1 Method for Monitoring Textile Fiber Quality, Analysis and Identification of Paper, Wood and Other Cellulose Containing Materials and US Patent Application Publication US20040152201A1, Method for Monitoring Textile Fiber Quality, Analysis and Identification of Paper, Wood, Grains, Foods and Other Cellulose Containing Materials Using Glycan Oligomer Analysis (Allen K. Murray). The acetic nitric reagent is used in addition to the dilute HCI extraction as described in the cited patent and patent application.

Abstract translation: 该专利申请是用于从含碳水化合物的材料中提取低聚糖和多糖的乙酸硝酸试剂（80％乙酸，1.8N硝酸）。 将材料用乙酸硝酸试剂在沸水浴中萃取不同时间，通常为30分钟。 然后将材料离心，然后将澄清的淡黄色上清液在减压下在Speed Vac中取干。 然后将干燥的残余物吸收在水中并离心以除去微粒。 然后通过具有集成电流检测的高pH阴离子交换色谱法分析所得上清液。 所得到的色谱图或峰下的积分区域是特定的材料来源的特征。 该方法可用于棉纤维，木材，纸张，纺织品或任何纤维素材料的分析。 该方法是用于表征这些材料的附加方法，如美国专利No.06562626B1中描述的用于监测纺织纤维质量，纸，木材和其它含纤维素材料的分析和鉴定的方法和美国专利申请公开US20040152201A1，纺织纤维监测方法 使用聚糖低聚物分析的纸，木材，谷物，食品和其他含纤维素材料的质量，分析和鉴定（Allen K. Murray）。 除了引用的专利和专利申请中所述的稀HCl萃取之外，还使用乙酸硝酸试剂。

公开(公告)号：WO2009047583A3

公开(公告)日：2009-09-17

申请号：PCT/IB2007004690

申请日：2007-01-04

Applicant: GLYCOZYME INC ,  MURRAY ALLEN K

Inventor： MURRAY ALLEN K

IPC: G01N33/46 ,  G01N30/96

CPC classification number: G01N33/362 ,  G01N30/96 ,  G01N2030/8809 ,  Y10T436/143333 ,  B01D15/363

Abstract: This patent application is for the use of acetic nitric reagent (80% acetic acid, 1.8 N nitric acid) for the extraction of oligosaccharides and polysaccharides from carbohydrate containing materials The material is extracted with the acetic nitric reagent in a boiling water bath for various periods of time, usually 30 minutes The material is then centrifuged and the clear, yellowish, supernatant is then taken to dryness in a Speed Vac under reduced pressure, The dry residue is then taken up in water and centrifuged to remove particulates The resulting supernatant is then analyzed by high pH anion exchange chromatography with integrated amperonetric detection The resulting chromatogram or the integrated areas under the peaks are then characteristic for that particular source of material.

Abstract translation: 该专利申请是使用乙酸硝酸试剂（80％乙酸，1.8N硝酸）从含碳水化合物的材料中提取寡糖和多糖。材料用乙酸硝酸试剂在沸水浴中萃取不同时期 的时间，通常为30分钟然后将材料离心，然后将透明，淡黄色的上清液在减压下在Speed Vac中将其干燥，然后将干燥的残余物溶于水中并离心除去颗粒。然后得到的上清液 通过高pH阴离子交换色谱法进行综合分析。所得到的色谱图或峰下的积分区域是特定的材料来源。

公开(公告)号：WO2003060227A2

公开(公告)日：2003-07-24

申请号：PCT/US2003/000753

申请日：2003-01-10

Applicant: GLYCOZYME, INC ,  MURRAY, Allen, K.

Inventor： MURRAY, Allen, K.

IPC: D06M

CPC classification number: D06M13/432 ,  D06M16/003 ,  D06M2101/06 ,  G01N33/36

Abstract: Specific extraction of the oligomers from cotton fibers can be: achieved by a 24-hr incubation at 37°C with trypsin, chymotrypsin, proteinase K or pepsin, followed by a second 24-hr incubation at 37°C with cellulase (Trichoderma reesei) or ß-glucosidase. Alternatively, samples were first subjected to cellulase or ß-glucosidase treatment followed by the protease. The residual material is then treated with 0.5N HCI at 100°C and the extracts analyzed. Fibers treated with cellulase: followed by protease disintegrated and appeared as a cloudy solution, while the fibers treated with protease followed by cellulase retained their structural identity. This analysis reveals striking differences between cotton fibers from different varieties with respect to their susceptibility to enzymatic degradation. This protocol can be used to identify biochemical characteristics, which can then be correlated with genetic markers for advances in plant breeding.

Abstract translation: 来自棉纤维的低聚物的特异性提取可以是：在37℃下用胰蛋白酶，胰凝乳蛋白酶，蛋白酶K或胃蛋白酶孵育24小时，然后在37℃下用纤维素酶（里氏木霉）培养第二次24小时， 或β-葡糖苷酶。 或者，首先对样品进行纤维素酶或β-葡糖苷酶处理，然后进行蛋白酶。 然后在100℃下用0.5N HCl处理残余物，分析提取物。 用纤维素酶处理的纤维：随后蛋白酶分解并显示为浑浊溶液，而用蛋白酶处理纤维素纤维素酶的纤维保持其结构同一性。 该分析揭示了来自不同品种的棉纤维与其对酶降解敏感性的显着差异。 该方案可用于鉴定生物化学特征，然后可将其与植物育种进展的遗传标记相关联。

公开(公告)号：WO2003060227A3

公开(公告)日：2003-07-24

申请号：PCT/US2003/000753

申请日：2003-01-10

Applicant: GLYCOZYME, INC ,  MURRAY, Allen, K.

Inventor： MURRAY, Allen, K.

IPC: D06M13/432

Abstract: Specific extraction of the oligomers from cotton fibers can be: achieved by a 24-hr incubation at 37°C with trypsin, chymotrypsin, proteinase K or pepsin, followed by a second 24-hr incubation at 37°C with cellulase (Trichoderma reesei) or ß-glucosidase. Alternatively, samples were first subjected to cellulase or ß-glucosidase treatment followed by the protease. The residual material is then treated with 0.5N HCI at 100°C and the extracts analyzed. Fibers treated with cellulase: followed by protease disintegrated and appeared as a cloudy solution, while the fibers treated with protease followed by cellulase retained their structural identity. This analysis reveals striking differences between cotton fibers from different varieties with respect to their susceptibility to enzymatic degradation. This protocol can be used to identify biochemical characteristics, which can then be correlated with genetic markers for advances in plant breeding.

公开(公告)号：WO2002086496A1

公开(公告)日：2002-10-31

申请号：PCT/US2001/012904

申请日：2001-04-20

Applicant: GLYCOZYME, INC. ,  MURRAY, Allen, K.

Inventor： MURRAY, Allen, K.

IPC: G01N33/36

CPC classification number: G01N33/36 ,  G01N30/02 ,  G01N33/343 ,  G01N2001/4033 ,  Y10T436/143333 ,  Y10T436/255 ,  B01D15/36

Abstract: A method of analyzing cell wall components based on a hot dilute acid extraction, followed by alcohol precipitation, of plant cellulosic materials such as cotton fibers or wood pulp. The extracts are analyzed by high pH anion exchange chromatography to separate and characterize the carbohydrates. This method extracts a characteristic series of carbohydrate multimers containing galactose, mannose and glucose. The pattern of multimers is indicative of origin of the cellulosic material ( e.g. , the plant species the material comes from) as well as quality and processing state of the material. The alcohol precipitation improves the discriminating powers of the analysis so that the species of origin of plant products can be identified.

Abstract translation: 一种分析基于热稀酸萃取，然后醇沉淀的植物纤维素材料如棉花纤维或木浆的细胞壁组分的方法。 通过高pH阴离子交换色谱分析提取物以分离和表征碳水化合物。 该方法提取含有半乳糖，甘露糖和葡萄糖的特征系列的碳水化合物多聚体。 多聚体的图案表示纤维素材料（例如，材料来自的植物物种）的起源以及材料的质量和加工状态。 酒精沉淀提高了分析的区别力，从而可以鉴定植物产品的来源。

公开(公告)号：WO1997006668A1

公开(公告)日：1997-02-27

申请号：PCT/US1996013200

申请日：1996-08-14

Applicant: GLYCOZYME, INC.

Inventor： GLYCOZYME, INC. ,  MURRAY, Allen, K.

IPC: A01G25/16

CPC classification number: G01N33/362 ,  A01G25/16 ,  D06M13/432 ,  D21C5/005 ,  G01N30/00 ,  G01N30/02 ,  Y10T436/143333 ,  Y10T436/25 ,  B01D15/36

Abstract: A method of detecting environmental stress in plants, particularly water stress in cotton plants is based on a cold water extraction of plant tissues such as cotton fibers. The extract is analyzed by high pH anion exchange chromatography to separate and characterize the saccharides, oligosaccharides and other glycoconjugates extracted by cold water. The extracted carbohydrates represent a uniquely sensitive means to detect environmental stress. Environmentally stressed plants show both a qualitative and quantitative alteration of the extracted carbohydrates. The alteration in extracted carbohydrates can be used to indicate when additional irrigation or other correction to the growth conditions is required.

Abstract translation: 检测植物中环境压力​​的方法，特别是棉花植物中的水分胁迫，是基于棉花纤维等植物组织的冷水提取。 通过高pH阴离子交换色谱分析提取物，分离和表征冷水提取的糖，寡糖和其他糖缀合物。 提取的碳水化合物是检测环境压力的唯一敏感手段。 环境胁迫的植物显示提取的碳水化合物的定性和定量变化。 提取的碳水化合物的改变可用于指示何时需要额外的灌溉或对生长条件的其他校正。