公开(公告)号：WO2014085612A1

公开(公告)日：2014-06-05

申请号：PCT/US2013/072275

申请日：2013-11-27

Applicant: INVISTA NORTH AMERICA S.Á R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P5/02 ,  C07C11/09

CPC classification number: C12P5/026 ,  Y02E50/343

Abstract: The document provides methods for biosynthesizing isobutene using one or more isolated enzymes such as one or more of an enoyl-CoA dehydratase, a 2-hydroxyacyl-CoA dehydratase , an isovaleryl-CoA / acyl-CoA dehydrogenase and a mevalonate diphosphate decarboxylase, or using recombinant host cells expressing one or more such enzymes.

Abstract translation: 该文献提供了使用一种或多种分离的酶如烯酰辅酶A脱水酶，2-羟基酰基辅酶A脱水酶，异戊酰辅酶A /酰基辅酶A脱氢酶和甲羟戊酸二磷酸脱羧酶之一或多种分离的酶生物合成异丁烯的方法，或使用 表达一种或多种这样的酶的重组宿主细胞。

公开(公告)号：WO2015021059A1

公开(公告)日：2015-02-12

申请号：PCT/US2014/049807

申请日：2014-08-05

Applicant: INVISTA NORTH AMERICA S.Á R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P5/02 ,  C12N15/66

CPC classification number: C12P5/026 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12Y208/02 ,  C12Y301/02 ,  C12Y301/02004 ,  C12Y401/01033 ,  C12Y402/01017 ,  C12Y402/01119

Abstract: The document provides methods for biosynthesizing isobutene using one or more isolated enzymes such as one or more of a hydratase such as an enzyme classified under EC 4.2.1.- and a decarboxylating thioesterase, or using recombinant host cells expressing one or more such enzymes.

Abstract translation: 该文献提供了使用一种或多种分离的酶如一种或多种水合酶如EC4.2.1.分类的酶和脱羧硫酯酶或使用表达一种或多种这样的酶的重组宿主细胞来生物合成异丁烯的方法。