公开(公告)号：WO2008066245A1

公开(公告)日：2008-06-05

申请号：PCT/KR2007/004827

申请日：2007-10-02

Applicant: KOREA OCEAN RESEARCH & DEVELOPMENT INSTITUTE ,  LEE, Jung Hyun ,  KANG, Sung Gyun ,  KIM, Sang Jin ,  KWON, Kae Kyoung ,  LEE, Hyun Sook ,  KIM, Yun Jae ,  RYU, Yong Gu ,  BAE, Seung Seob ,  LIM, Jae Kyu ,  JEON, Jung Ho ,  CHO, Yo Na ,  JEONG, Insoon ,  KWON, Suk Tae ,  CHA, Sun Shin

Inventor： LEE, Jung Hyun ,  KANG, Sung Gyun ,  KIM, Sang Jin ,  KWON, Kae Kyoung ,  LEE, Hyun Sook ,  KIM, Yun Jae ,  RYU, Yong Gu ,  BAE, Seung Seob ,  LIM, Jae Kyu ,  JEON, Jung Ho ,  CHO, Yo Na ,  JEONG, Insoon ,  KWON, Suk Tae ,  CHA, Sun Shin

IPC: C12N9/12

CPC classification number: C12N9/1252

Abstract: The present invention relates to mutant DNA polymerases and their genes isolated from Thermococcus sp. More specifically, the present invention relates to mutant DNA polymerases which are originally isolated from Thermococcus sp NAl. strain and produced by site-specific mutagenesis, their amino acid sequences, genes encoding said mutant DNA polymerases, their nucleic acids sequences, recombinant vectors containing said nucleic acids sequences, host cells transformed with thereof and methods for producing mutant DNA polymerase protein by using thereof. As mutant DNA polymerases according to the present invention have increased processivity by site-specific mutagenesis on exonuclease active site compared to wild type DNA polymerase, the present invention is broadly applicable for PCR in various molecular genetic technologies.

Abstract translation: 本发明涉及从Thermococcus sp。分离的突变型DNA聚合酶及其基因。 更具体地，本发明涉及最初从Thermococcus sp NA1分离的突变型DNA聚合酶。 菌株并通过位点特异性诱变产生，其氨基酸序列，编码所述突变型DNA聚合酶的基因，其核酸序列，含有所述核酸序列的重组载体，用其转化的宿主细胞和通过使用其产生突变型DNA聚合酶蛋白的方法 。 由于本发明的突变型DNA聚合酶与野生型DNA聚合酶相比，通过对外切核酸酶活性位点的位点特异性诱变具有增加的进行性，因此本发明广泛适用于各种分子遗传技术中的PCR。

公开(公告)号：WO2008066350A1

公开(公告)日：2008-06-05

申请号：PCT/KR2007/006150

申请日：2007-11-30

Applicant: KOREA OCEAN RESEARCH & DEVELOPMENT INSTITUTE ,  LEE, Jung Hyun ,  KANG, Sung Gyun ,  KIM, Sang Jin ,  KWON, Kae Kyoung ,  LEE, Hyun Sook ,  KIM, Yun Jae ,  RYU, Yong Gu ,  BAE, Seung Seob ,  LIM, Jae Kyu ,  JEON, Jung Ho ,  CHO, Yo Na ,  JEONG, Insoon ,  KWON, Suk Tae ,  CHA, Sun Shin

Inventor： LEE, Jung Hyun ,  KANG, Sung Gyun ,  KIM, Sang Jin ,  KWON, Kae Kyoung ,  LEE, Hyun Sook ,  KIM, Yun Jae ,  RYU, Yong Gu ,  BAE, Seung Seob ,  LIM, Jae Kyu ,  JEON, Jung Ho ,  CHO, Yo Na ,  JEONG, Insoon ,  KWON, Suk Tae ,  CHA, Sun Shin

IPC: C07K14/195

CPC classification number: C12N9/16

Abstract: The present invention relates to a novel dITPase, protein for enhancing DNA polymerase activity, and genes encoding it. More specifically, the present invention relates to a dITPase isolated from Thermococcus sp NAl. strain. Also, the present invention provides gene fragments encoding said protein, recombinant vectors containing thereof, host cells transformed with thereof and methods for producing dITPase using said host cells.

Abstract translation: 本发明涉及一种新的dITPase，用于增强DNA聚合酶活性的蛋白质和编码它的基因。 更具体地说，本发明涉及从Thermococcus sp NA1分离的dITPase。 应变。 此外，本发明提供编码所述蛋白质的基因片段，含有其的重组载体，用其转化的宿主细胞和使用所述宿主细胞产生dITPase的方法。