公开(公告)号：WO2003048178A3

公开(公告)日：2003-06-12

申请号：PCT/GB2002/005375

申请日：2002-11-28

Applicant: AMERSHAM BIOSCIENCES UK LIMITED ,  PICKERING, Lea ,  ODEDRA, Raj ,  SIMMONDS, Adrian ,  JOHNSON, Karin, Sofia, Helena

Inventor： PICKERING, Lea ,  ODEDRA, Raj ,  SIMMONDS, Adrian ,  JOHNSON, Karin, Sofia, Helena

IPC: C07H19/00

Abstract: The invention relates to nucleosides comprising a reporter moiety which also functions to limit polymerase activity, characterised in that the reporter moiety is attached to the nucleoside through a linkage group cleavable by a hydrolase enzyme wherein the hydrolase enzyme is selected from the group consisting of esterases, phosphatases, peptidases, penicillin amidases, glycosidases and phosphorylases.

公开(公告)号：WO2003048178A2

公开(公告)日：2003-06-12

申请号：PCT/GB2002/005375

申请日：2002-11-28

Applicant: AMERSHAM BIOSCIENCES UK LIMITED ,  JOHNSON, Karin, Sofia, Helena ,  PICKERING, Lea ,  ODEDRA, Raj ,  SIMMONDS, Adrian

Inventor： PICKERING, Lea ,  ODEDRA, Raj ,  SIMMONDS, Adrian

IPC: C07H19/00

CPC classification number: C07H19/06 ,  C07H19/10 ,  C09B11/08 ,  C12Q1/6869 ,  C12Q2525/117

Abstract: The invention relates to nucleosides comprising a reporter moiety which also functions to limit polymerase activity, characterised in that the reporter moiety is attached to the nucleoside through a linkage group cleavable by a hydrolase enzyme wherein the hydrolase enzyme is selected from the group consisting of esterases, phosphatases, peptidases, penicillin amidases, glycosidases and phosphorylases.

Abstract translation: 本发明涉及包含报道部分的核苷，其也起到限制聚合酶活性的作用，其特征在于报道部分通过可由水解酶切割的连接基团与核苷连接，其中水解酶选自酯酶， 磷酸酶，肽酶，青霉素酰胺酶，糖苷酶和磷酸酶。