公开(公告)号：WO2011159797A3

公开(公告)日：2012-04-26

申请号：PCT/US2011040519

申请日：2011-06-15

Applicant: CELLULAR DYNAMICS INT INC ,  NUWAYSIR EMILE ,  KENDRICK-PARKER CHRIS ,  SEAY NICHOLAS

Inventor： NUWAYSIR EMILE ,  KENDRICK-PARKER CHRIS ,  SEAY NICHOLAS

IPC: C12N5/074 ,  C12N5/10 ,  C12N15/65 ,  C12N15/85 ,  C12Q1/02 ,  G01N33/15

CPC classification number: G01N33/5023 ,  G01N33/5014 ,  G01N33/5041 ,  G01N33/5073

Abstract: The invention generally features methods for providing engineered pluripotent stem cells that can be used to study biological response and pathways, including differentiation and drug effects. For example, these cells are provided comprising two or more exogenous expression cassettes including a selectable or screenable marker under the control of different condition-responsive regulatory elements, such as differentiation-responsive promoters or regulatory element of a receptor, drug target, drug metabolizing enzyme or signaling pathway gene. Also provided are sets of stem cell lines each comprising a different exogenous expression cassette including a selectable or screenable marker under the control of a different condition-responsive regulatory element.

Abstract translation: 本发明通常具有用于提供可用于研究生物反应和途径（包括分化和药物作用）的工程多能干细胞的方法。 例如，提供这些细胞，其包含两种或多种外源性表达盒，其包括在不同的条件反应性调节元件的控制下的可选择或可筛选标记，例如受体的分化响应性启动子或调节元件，药物靶标，药物代谢酶 或信号通路基因。 还提供了一组干细胞系，其各自包含不同的外源表达盒，其包含在不同的条件响应性调节元件的控制下的可选择或可筛选标记。

公开(公告)号：WO2011159797A2

公开(公告)日：2011-12-22

申请号：PCT/US2011/040519

申请日：2011-06-15

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC. ,  NUWAYSIR, Emile ,  KENDRICK-PARKER, Chris ,  SEAY, Nicholas

Inventor： NUWAYSIR, Emile ,  KENDRICK-PARKER, Chris ,  SEAY, Nicholas

IPC: C12N5/074 ,  C12N5/10 ,  C12N15/65 ,  C12N15/85 ,  C12Q1/02 ,  G01N33/15

CPC classification number: G01N33/5023 ,  G01N33/5014 ,  G01N33/5041 ,  G01N33/5073

Abstract: The invention generally features methods for providing engineered pluripotent stem cells that can be used to study biological response and pathways, including differentiation and drug effects. For example, these cells are provided comprising two or more exogenous expression cassettes including a selectable or screenable marker under the control of different condition-responsive regulatory elements, such as differentiation-responsive promoters or regulatory element of a receptor, drug target, drug metabolizing enzyme or signaling pathway gene. Also provided are sets of stem cell lines each comprising a different exogenous expression cassette including a selectable or screenable marker under the control of a different condition-responsive regulatory element.

Abstract translation: 本发明通常具有用于提供可用于研究生物反应和途径（包括分化和药物作用）的工程多能干细胞的方法。 例如，提供这些细胞包含两种或更多种外源性表达盒，其包括在不同的条件反应性调控元件的控制下的可选择或可筛选标记，例如受体的分化反应性启动子或调节元件，药物靶标，药物代谢酶 或信号通路基因。 还提供了一组干细胞系，其各自包含不同的外源表达盒，其包含在不同的条件响应性调节元件的控制下的可选择或可筛选标记。