公开(公告)号：WO2009067757A1

公开(公告)日：2009-06-04

申请号：PCT/AU2008/001765

申请日：2008-11-28

Applicant: CYTOMATRIX PTY LTD ,  KIRKLAND, Mark, Alexander ,  GOUGH, Tamara, Jane

Inventor： KIRKLAND, Mark, Alexander ,  GOUGH, Tamara, Jane

IPC: A61K38/00 ,  A61K38/18 ,  C12N15/07 ,  C12N15/08

CPC classification number: A61K38/1709 ,  A61K38/1825 ,  A61K2300/00

Abstract: The invention relates to a method of inducing pluripotency in a responsive mammalian cell, which comprises introducing into the cell an effective amount for initiating pluripotency within the cell of Oct4 protein or a functionally equivalent analogue, variant or fragment thereof. The invention also relates to a method of treatment and/or prophylaxis of a degenerative disease or injury in a mammal, which comprises removing from the mammal one or more responsive cells and culturing the cells in a suitable medium, introducing into the cells an effective amount of Oct4 protein or a functionally equivalent analogue, variant or fragment thereof and subsequently returning the cells to the patient. A further aspect of the invention relates to a method of treatment and/or prophylaxis of a degenerative disease or injury in a mammal, which comprises introducing into responsive cells of the patient an effective amount of Oct4 protein or a functionally equivalent analogue, variant or fragment thereof.

Abstract translation: 本发明涉及在响应性哺乳动物细胞中诱导多能性的方法，其包括向Oct4蛋白或其功能等同的类似物，变体或片段的细胞内引入有效量的起始多能性的有效量。 本发明还涉及治疗和/或预防哺乳动物的退行性疾病或损伤的方法，其包括从哺乳动物中除去一种或多种反应性细胞并在合适的培养基中培养细胞，向细胞中引入有效量 的Oct4蛋白或其功能等同的类似物，变体或片段，随后将细胞返回给患者。 本发明的另一方面涉及一种治疗和/或预防哺乳动物的退行性疾病或损伤的方法，其包括将有效量的Oct4蛋白或功能等同的类似物，变体或片段引入患者的有效量的患者的反应性细胞中 它们。

公开(公告)号：WO2009067756A1

公开(公告)日：2009-06-04

申请号：PCT/AU2008/001764

申请日：2008-11-28

Applicant: CYTOMATRIX PTY LTD ,  KIRKLAND, Mark, Alexander ,  GOUGH, Tamara, Jane

Inventor： KIRKLAND, Mark, Alexander ,  GOUGH, Tamara, Jane

IPC: A61K38/00 ,  A61K38/18 ,  C12N15/07 ,  C12N15/08

CPC classification number: A61K38/1825 ,  A61K38/162 ,  A61K38/1709 ,  A61K38/1808 ,  A61K38/30 ,  A61K2300/00

Abstract: The invention relates to a method of inducing pluripotency in a responsive mammalian cell, which comprises introducing into the cell an effective amount for initiating pluripotency within the cell of Sox2 protein or a functionally equivalent analogue, variant or fragment thereof. The invention also relates to a method of treatment and/or prophylaxis of a degenerative disease or injury in a mammal, which comprises removing from the mammal one or more responsive cells and culturing the cells in a suitable medium, introducing into the cells an effective amount of Sox2 protein or a functionally equivalent analogue, variant or fragment thereof and subsequently returning the cells to the patient. In a further embodiment the invention relates to a method of treatment and/or prophylaxis of a degenerative disease or injury in a mammal, which comprises introducing into responsive cells of the mammal an effective amount of Sox2 protein or a functionally equivalent analogue, variant or fragment thereof.

Abstract translation: 本发明涉及在响应性哺乳动物细胞中诱导多能性的方法，其包括向Sox2蛋白或其功能等同的类似物，变体或片段的细胞内引入有效量的起始多能性的有效量。 本发明还涉及治疗和/或预防哺乳动物的退行性疾病或损伤的方法，其包括从哺乳动物中除去一种或多种反应性细胞并在合适的培养基中培养细胞，向细胞中引入有效量 的Sox2蛋白或其功能等同的类似物，变体或片段，随后将细胞返回给患者。 在另一个实施方案中，本发明涉及治疗和/或预防哺乳动物的退行性疾病或损伤的方法，其包括将有效量的Sox2蛋白或功能等同的类似物，变体或片段引入哺乳动物的反应性细胞中 它们。

公开(公告)号：WO2008151387A1

公开(公告)日：2008-12-18

申请号：PCT/AU2008/000862

申请日：2008-06-13

Applicant: CYTOMATRIX PTY LTD ,  KIRKLAND, Mark, Alexander ,  VO, Liem, Thanh

Inventor： KIRKLAND, Mark, Alexander ,  VO, Liem, Thanh

IPC: C12N5/06 ,  C12N5/08 ,  A61K38/17

CPC classification number: A61K38/1709 ,  A61K35/34 ,  C12N5/0657 ,  C12N2501/60 ,  C12N2510/00

Abstract: In one embodiment the invention relates to a method of initiating cardiomyocyte differentiation in a responsive mammalian cell, which comprises introducing into the cell an effective amount for initiating cardiomyocyte differentiation within the cell of CSX/Nkx2.5 protein or a functionally equivalent analogue, variant or fragment thereof. In another embodiment the invention relates to a method of treatment of a patient suffering from or prone to suffer from heart failure or ischaemic heart disease, which comprises removing from the patient one or more responsive cells and culturing the cells in a suitable medium, introducing into the cells an effective amount for initiating cardiomyocyte differentiation of CSX/Nkx2.5 protein or a functionally equivalent analogue, variant or fragment thereof and subsequently returning the cells or cells derived from them to the patient. In a further embodiment the invention relates to a method of treatment of a patient suffering from or prone to suffer from heart failure or ischaemic heart disease, which comprises introducing into responsive cells of the patient an effective amount of CSX/Nkx2.5 protein or a functionally equivalent analogue, variant or fragment thereof.

Abstract translation: 在一个实施方案中，本发明涉及在响应性哺乳动物细胞中引发心肌细胞分化的方法，其包括向CSX / Nkx2.5蛋白的细胞内引入有效量的起始心肌细胞分化或功能等同的类似物，变体或 片段。 在另一个实施方案中，本发明涉及治疗患有或易于患有心力衰竭或缺血性心脏病的患者的方法，其包括从患者中除去一种或多种应答细胞并在合适的培养基中培养细胞，将其引入 所述细胞是用于启动CSX / Nkx2.5蛋白或其功能等同的类似物，变体或片段的心肌细胞分化的有效量，随后将从其衍生的细胞或细胞返回给患者。 在另一个实施方案中，本发明涉及治疗患有或易于患有心力衰竭或缺血性心脏病的患者的方法，其包括将有效量的CSX / Nkx2.5蛋白或 功能等同的类似物，变体或片段。