公开(公告)号：WO2010139948A3

公开(公告)日：2010-12-09

申请号：PCT/GB2010/001083

申请日：2010-06-02

Applicant: MEDICAL RESEARCH COUNCIL ,  NORTH CAROLINA STATE UNIVERSITY ,  NGUYEN, Duy, P. ,  NEUMANN, Heinz ,  DEITERS, Alexander ,  CHIN, Jason ,  LUSIC, Hrvoje

Inventor： NGUYEN, Duy, P. ,  NEUMANN, Heinz ,  DEITERS, Alexander ,  CHIN, Jason ,  LUSIC, Hrvoje

IPC: C12N15/67

Abstract: The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.

公开(公告)号：WO2010139948A2

公开(公告)日：2010-12-09

申请号：PCT/GB2010001083

申请日：2010-06-02

Applicant: MEDICAL RES COUNCIL ,  UNIV NORTH CAROLINA STATE ,  NGUYEN DUY P ,  NEUMANN HEINZ ,  DEITERS ALEXANDER ,  CHIN JASON ,  LUSIC HRVOJE

Inventor： NGUYEN DUY P ,  NEUMANN HEINZ ,  DEITERS ALEXANDER ,  CHIN JASON ,  LUSIC HRVOJE

IPC: C12N15/70

CPC classification number: C12N15/67 ,  C07C233/49 ,  C07C271/22 ,  C12N9/93 ,  C12P21/02

Abstract: The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.

Abstract translation: 本发明涉及一种制备包含正交官能团的多肽的方法，所述正交官能团由脂肪族氨基酸或氨基酸衍生物构成，所述方法包括提供宿主细胞; 提供编码目的多肽的核酸; 提供与所述宿主细胞正交的tRNA-tRNA合成酶对; 添加包含所述正交官能团的氨基酸或氨基酸衍生物，其中所述氨基酸或氨基酸衍生物是所述正交tRNA合成酶的底物，其中所述氨基酸或氨基酸衍生物具有脂族碳骨架; 并孵育以允许所述氨基酸或氨基酸衍生物通过正交的tRNA-tRNA合成酶对引入目的多肽。 本发明还涉及某些氨基酸，以及包含其的多肽。