公开(公告)号：WO2023060041A1

公开(公告)日：2023-04-13

申请号：PCT/US2022/077467

申请日：2022-10-03

申请人： DAY, Alexander ,  MULLIN, Bradford

发明人： DAY, Alexander ,  MULLIN, Bradford

IPC分类号： C12N15/88 ,  C07K14/51 ,  A61K38/18 ,  A61K48/00 ,  A61K47/69

摘要： Methods and treatments including preparing a synthetic messenger ribonucleic acid (mRNA) encoding at least for a bone morphogenic protein (BMP) and/or a BMP receptor. The synthetic mRNA is lipid-solubilized in a lipid or lipid derivative carrier. Intraoperatively, a bone harvest sample is obtained and incubated with the lipid-solubilized mRNA. The bone harvest sample internalizes at least a portion of the lipid-solubilized mRNA, thereby forming activated BMP and/or BMPR material. The activated BMP and/or BMPR material may be delivered intraoperatively to a bone fusion bed.