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公开(公告)号：WO9936556A3
公开(公告)日：1999-10-14
申请号：PCT/NL9900021
申请日：1999-01-15
申请人： NL ZUIVELONDERZOEK INST , HOLS PASCAL , KLEEREBEZEM MICHIEL , KUIPERS OSCAR PAUL , FERAIN THIERRY , DELCOUR JEAN MARCELIN ALAIN MA , VOS WILLEM MEINDERT DE
发明人： HOLS PASCAL , KLEEREBEZEM MICHIEL , KUIPERS OSCAR PAUL , FERAIN THIERRY , DELCOUR JEAN MARCELIN ALAIN MA , DE VOS WILLEM MEINDERT
CPC分类号： C12P13/06
摘要： The present invention relates to a process for preparing alanine, especially L-alanine, by culturing a genetically modified microorganism that is able to produce alanine from glucose, characterized in that the microorganism used is one whose native sugar metabolism has been diverted to alanine formation, especially L-alanine formation, in such a manner that essentially more than 40 %, preferably more than 60 % and more preferably more than 75 % of the carbon consumed from the medium is converted into alanine, especially via pyruvate. The microorganism is preferably a homofermentative microorganism, especially a lactic acid bacterium, more particularly (a strain of) L. lactis, and is preferably a food-grade microorganism. The invention can be used for both the in situ and the stereospecific preparation of alanine. The invention also relates to the microorganisms and recombinant DNA molecules that can be used in the invention and to the alanine and alanine-containing (food) products obtained according to the invention.
摘要翻译： 本发明涉及通过培养能够从葡萄糖生产丙氨酸的遗传修饰的微生物来制备丙氨酸，特别是L-丙氨酸的方法，其特征在于使用的微生物是将天然糖代谢转移到丙氨酸形成的微生物， 特别是L-丙氨酸形成，使得从培养基中消耗的碳的基本上超过40％，优选大于60％，更优选大于75％被转化为丙氨酸，特别是通过丙酮酸盐。 微生物优选是均匀发酵微生物，特别是乳酸菌，更特别是（乳酸乳杆菌）菌株，优选食品级微生物。 本发明可用于丙氨酸的原位和立体特异性制备。 本发明还涉及可用于本发明的微生物和重组DNA分子以及根据本发明获得的含丙氨酸和丙氨酸（食品）产品。
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公开(公告)号：WO9102064A3
公开(公告)日：1991-07-25
申请号：PCT/EP9001302
申请日：1990-08-02
发明人： VOS PETRUS ANTONIUS JOSEPHINA , SIEZEN ROELANT JAN , DE VOS WILLEM MEINDERT , KOK JAN , VENEMA GERHARDUS , HAANDRIKMAN ALFRED JACQUES
IPC分类号： C12N15/09 , A23L27/20 , A23L27/21 , C12N1/21 , C12N9/52 , C12N15/57 , C12R1/225 , A23L1/226 , C12N15/74
CPC分类号： C12N9/52 , C07K2319/00
摘要： The invention relates to DNA fragments containing at least one 'mutant protease gene', which 'mutant protease gene' is understood to mean: a gene made up of sections of parent protease genes of several lactococcal strains, in particular of the parent protease genes of L.lactis subsp. cremoris Wg2 and L.lactis subsp. cremoris SK11, as well as a parent protease gene of a lactococcal strain, in particular the parent protease gene of L.lactis subsp. cremoris Wg2 or the parent protease gene of L.lactis subsp. cremoris SK11, the DNA sequence of which has been altered in a manner such that in the mutant protease for which the gene codes: (a) an amino acid other than that of the 'wild-type' protease is present at at least one site, and/or (b) at at least one site within the first 1350 residues of the amino acid sequence, calculated from the N-terminus, at least one amino acid of the 'wild-type' protease is missing and/or one or more amino acids have been inserted, or (c) at at least two sites separated from each other, one or more amino acids are missing and/or one or more amino acids have been inserted, the cloning vectors containing such a DNA fragment, the transformed host strains containing such a DNA fragment or vector, the mutant proteases obtainable as well as the foodstuffs and flavourings produced with the host strains or mutant proteases meant above.
摘要翻译： 本发明涉及含有至少一种“突变蛋白酶基因”的DNA片段，其中“突变蛋白酶基因”理解为指由几种乳球菌菌株的亲本蛋白酶基因片段组成的基因，特别是由 L.lactis subsp。 cremoris Wg2和L.lactis subsp。 cremoris SK11以及乳球菌菌株的亲本蛋白酶基因，特别是乳酸乳球菌（L.lactis subsp。）的亲本蛋白酶基因。 cremoris Wg2或L.lactis subsp。的亲本蛋白酶基因。 （cremoris）SK11，其DNA序列已被改变，使得在基因编码的突变型蛋白酶中：（a）除“野生型”蛋白酶之外的氨基酸存在于至少一个位点 ，和/或（b）在从N端计算的氨基酸序列的前1350个残基内的至少一个位点处，缺失“野生型”蛋白酶的至少一个氨基酸和/或 已经插入了更多的氨基酸，或（c）在彼此分开的至少两个位点上，一个或多个氨基酸缺失和/或一个或多个氨基酸已被插入，含有这种DNA片段的克隆载体， 包含这种DNA片段或载体的转化宿主菌株，可获得的突变蛋白酶以及用上述宿主菌株或突变蛋白酶产生的食品和调味品。
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