公开(公告)号：WO1991009871A1

公开(公告)日：1991-07-11

申请号：PCT/US1990007619

申请日：1990-12-21

申请人： SERAGEN INCORPORATED

发明人： SERAGEN INCORPORATED ,  MURPHY, John, R.

IPC分类号： C07K13/00

CPC分类号： C07K14/005 ,  C07K14/245 ,  C07K14/25 ,  C07K14/28 ,  C07K14/34 ,  C07K14/415 ,  C07K14/55 ,  C07K14/575 ,  C07K2319/00 ,  C07K2319/55 ,  C07K2319/75 ,  C12N9/0071 ,  C12N9/2408 ,  C12N15/62 ,  C12N2740/16222 ,  C12N2770/24022 ,  C12Y114/16001 ,  G01N33/56966 ,  G01N33/68

摘要： A hybrid molecule including a first part, a second part and a third part connected by covalent bonds, (a) the first part including a portion of the binding domain of a cell-binding ligand, which portion is able to cause the hybrid molecule of the invention to bind to an animal cell; (b) the second part including a portion of a translocation domain of a protein, which portion is capable of translocating the third part across the cytoplasmic membrane of the cell; and (c) the third part including a chemical entity to be introduced into the cell, provided that (i) the hybrid molecule is produced by expression of a recombinant DNA molecule encoding the hybrid molecule, and (ii) the second and third parts are not segments of the same naturally- occurring polypeptide toxin; a recombinant DNA molecule encoding the hybrid molecule; and a method of producing the hybrid molecule.