公开(公告)号：WO2014076286A1

公开(公告)日：2014-05-22

申请号：PCT/EP2013/074113

申请日：2013-11-18

Applicant: ALERE TECHNOLOGIES GMBH

Inventor： LABUGGER, Ines ,  DEES, Stefan ,  ERMANTRAUT, Eugen

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6851 ,  C12Q2600/118 ,  C12Q2600/158 ,  Y02A50/55 ,  Y02A50/58

Abstract: Described herein is a method and assay of detecting the presence of a polynucleotide in a bodily fluid obtained from a subject treated with an anti-pathogenic agent comprising isolating the polynucleotide from a first and a second sample of a bodily fluid, amplifying the polynucleotide, and determining the polynucleotide, wherein the polynucleotide is a pathogen polynucleotide. Further provided is a method for diagnosing a pathogen infection in a subject, for detecting a pathogen infection in a subject treated with an anti-pathogenic agent, and for detecting the presence and/or genotype of a pathogen in a bodily fluid.

Abstract translation: 本文描述的是检测从用抗病原体处理的受试者获得的体液中多核苷酸的存在的方法和测定法，其包括从体液的第一和第二样品分离多核苷酸，扩增多核苷酸，以及 确定所述多核苷酸，其中所述多核苷酸是病原体多核苷酸。 还提供了用于诊断受试者中病原体感染的方法，用于检测用抗病原体治疗的受试者中的病原体感染，以及用于检测体液中病原体的存在和/或基因型。