公开(公告)号：WO2020247790A1

公开(公告)日：2020-12-10

申请号：PCT/US2020/036380

申请日：2020-06-05

Applicant: AMGEN INC.

Inventor： BONDARENKO, Pavel ,  CROUSE-ZEINEDDINI, Jill A. ,  KUHNS, Scott Thomas ,  NICHOLS, Andrew ,  XIAO, Gang ,  SHI, Rachel Liuqing

IPC: G01N33/68

Abstract: Provided herein are methods of identifying structures, e.g ., attributes, of a therapeutic protein or a target that affect an interaction between the therapeutic protein and the target. In exemplary embodiments, the method comprises: (a) applying a stress to a first sample comprising therapeutic proteins or targets; (b) contacting the first sample with a second sample comprising targets or therapeutic proteins to form a mixture comprising (i) therapeutic protein- target complexes, (ii) unbound therapeutic proteins, and (iii) unbound targets; and (c) separating the mixture into at least two fractions, wherein an unbound fraction comprises unbound therapeutic proteins or unbound targets and a bound fraction comprises therapeutic protein-target complexes; and (d) for each of the unbound fraction and bound fraction, identifying and quantifying the abundance of the structures, e.g ., attribute, present on a species of the therapeutic protein or target.