公开(公告)号：WO2022225921A1

公开(公告)日：2022-10-27

申请号：PCT/US2022/025340

申请日：2022-04-19

Applicant: AMGEN INC.

Inventor： GONG, Danyang ,  ESTES, Bram ,  WANG, Zhulun ,  GARCES, Fernando

IPC: C07K16/00 ,  C07K16/46

Abstract: The clinical potential of multispecific antibodies like bispecific and trispecific antibodies shows great promise for targeting complex diseases. However, the generation of those molecules presents great challenges particularly in regard to achieving acceptable expression levels free from mis-paired polypeptides. The presently claimed invention is directed to multispecific antigen binding proteins which improve upon existing charge pair technologies by redistributing the engineered charges within the CH3 regions of a heteromultimer.

公开(公告)号：WO2022076474A2

公开(公告)日：2022-04-14

申请号：PCT/US2021/053650

申请日：2021-10-05

Applicant: AMGEN INC.

Inventor： GONG, Danyang ,  GARCES, Fernando ,  WANG, Zhulun

IPC: C07K16/00 ,  C12N15/09 ,  C07K2317/14 ,  C07K2317/31 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/92

Abstract: The ability to generate a single antibody-based construct that can recognize multiple targets simultaneously, is paramount to advance many therapeutics candidates to clinic. Often, this implies extensive protein design with vary degrees of success. In the case of multispecific antibody constructs, there are multiple modalities from which to choose and often multiple antigen binders as well. Described here is the discovery of new methods to optimally pair antigen binders with the proper format, including the selection of common light chains.