公开(公告)号：WO2023275819A1

公开(公告)日：2023-01-05

申请号：PCT/IB2022/056114

申请日：2022-06-30

Applicant: AZIENDA UNITA' SANITARIA LOCALE DELLA ROMAGNA

Inventor： BONDIOLI, Elena ,  PURPURA, Valeria ,  MELANDRI, Davide

IPC: A01N1/02 ,  A01P1/00 ,  A01N25/02 ,  A01N33/12 ,  A01N47/44 ,  A01N1/021 ,  A01N1/0215

Abstract: The present invention relates to a method of preserving a tissue, preferably at room temperature, wherein the tissue is preserved for a period of up to 3 years in a solution comprising: at least one polymer containing at least one positively charged quaternary ammonium, at least one antiseptic agent chosen from polyhexaalkylene biguanidine and/or glycerol, and at least one surfactant. The tissue preserved with the method of the invention is chosen from: skin tissue or skin, De-epidermized Dermis (Ded), Decellularized Dermis (Derma DEC or Ded DEC), a placental tissue or a placental tissue derivative, preferably amniotic membrane, preferably human (HAM), a musculoskeletal tissue, preferably chosen from: tendons, bones, cartilage, nerves, an eye tissue, preferably chosen from: cornea and eyeball, a tissue chosen from: a valve, a vessel, aorta and an artery, preferably a femoral artery, a tissue belonging to the male and/or female genital apparatus, preferably chosen from foreskin, ovary, uterus.

公开(公告)号：WO2023275821A1

公开(公告)日：2023-01-05

申请号：PCT/IB2022/056117

申请日：2022-06-30

Applicant: AZIENDA UNITA' SANITARIA LOCALE DELLA ROMAGNA

Inventor： BONDIOLI, Elena ,  PURPURA, Valeria ,  MELANDRI, Davide

IPC: A61L27/36 ,  A61L2430/40 ,  A61L27/3633 ,  A61L27/3687

Abstract: The invention relates to a method for decellularizing a tissue and/or organ that comprises immersing an isolated tissue and/or organ in an enzyme solution and a step of irradiating the tissue with ionizing electromagnetic radiation. The tissue and/or organ thus treated are immersed in an aqueous solution comprising: at least one polymer containing at least one positively charged quaternary ammonium, at least one antiseptic agent chosen from polyhexaalkylene biguanidine and/or glycerol, and at least one surfactant and the tissue and/or organ are maintained in the aqueous solution for at least 1 hour. The tissue that can be decellularized with the method of the invention is skin, consisting of the epidermal layer containing all of the cellular components making it up and a thin layer of the underlying dermis (dermo-epidermal tissue); and the de-epidermized dermis (Ded), consisting of dermis without the overlying epidermal layer.