公开(公告)号：WO2019149723A1

公开(公告)日：2019-08-08

申请号：PCT/EP2019/052183

申请日：2019-01-30

Applicant: BACHEM HOLDING AG

Inventor： EISENHUTH, Ralf ,  LOIDL, Günther ,  SAMSON, Daniel ,  SCHÖNLEBER, Ralph O.

IPC: C07K14/605 ,  C07K1/20

CPC classification number: C07K14/605 ,  C07K1/20 ,  C07K2319/20

Abstract: The present invention refers to a method of purifying a glucagon peptide, the method comprising a two dimensional reversed phase high performance liquid chromatography protocol, wherein the first step is carried out at using a mobile phase comprising a triethylammonium phosphate (TEAP) buffer and acetonitrile, and the second step is carried out at using a mobile phase comprising aqueous acetic acid and acetonitrile.

公开(公告)号：WO2020212477A1

公开(公告)日：2020-10-22

申请号：PCT/EP2020/060691

申请日：2020-04-16

Applicant: BACHEM HOLDING AG

Inventor： EISENHUTH, Ralf ,  SAMSON, Daniel ,  SCHÖNLEBER, Ralph O. ,  MARCHETTI, Patrizia

IPC: C07K1/113 ,  C07K7/16 ,  C07K14/655

Abstract: A method and apparatus for the manufacture of disulfide bonded peptides is provided, wherein a solution of an oxidizing agent and a solution of a peptide comprising at least two sulfhydryl groups are added simultaneously into a reaction vessel under such conditions that the average concentration of the oxidizing agent inside the reaction vessel is essentially zero during simultaneous addition.

公开(公告)号：WO2017162653A1

公开(公告)日：2017-09-28

申请号：PCT/EP2017/056668

申请日：2017-03-21

Applicant: BACHEM HOLDING AG

Inventor： STADELMAIER, Andreas ,  SCHÖNLEBER, Ralph O. ,  SAMSON, Daniel ,  DETTNER, Frank

IPC: C07K14/605 ,  B01D15/32 ,  C07K1/00

Abstract: The present invention refers to a method of purifying a glucagon-like peptide 1 analogs, the method comprising a two dimensional reversed phase high performance liquid chromatography protocol, wherein the first step is carried out at a pH value between 7.0 to 7.8 using a mobile phase comprising a phosphate buffer and acetonitrile, and the second step is carried out at a pH value below 3.0 using a mobile phase comprising trifluoroacetic acid and acetonitrile.

Abstract translation: 本发明涉及一种纯化胰高血糖素样肽1类似物的方法，所述方法包括二维反相高效液相色谱方案，其中所述第一步骤在pH值 使用包含磷酸盐缓冲液和乙腈的流动相在7.0至7.8之间，并且使用包含三氟乙酸和乙腈的流动相在pH值低于3.0的条件下进行第二步骤。

公开(公告)号：WO2022195111A1

公开(公告)日：2022-09-22

申请号：PCT/EP2022/057241

申请日：2022-03-18

Applicant: BACHEM HOLDING AG

Inventor： EISENHUTH, Ralf ,  HAUG, Karl Rüdiger ,  SAMSON, Daniel ,  BERTHELMANN, Arne ,  CREUSEN, Guido

IPC: C07H1/00 ,  C07H1/02 ,  C07H21/00

Abstract: An improved protocol for the synthesis of oligonucleotides is provided, which reduces the level of depurination. A liquid composition for the cleavage of a di(p-methoxyphenyl) phenylmethyl protecting group from a hydroxyl group and the use thereof in chemical synthesis of oligonucleotides is disclosed.