公开(公告)号：WO2013147610A3

公开(公告)日：2014-01-09

申请号：PCT/NL2013050239

申请日：2013-04-02

Applicant: BOTMA JETZE ,  KLEEFSTRA MARTIJN ,  VAN DER ZEE TINO WALTER

Inventor： BOTMA JETZE ,  KLEEFSTRA MARTIJN ,  VAN DER ZEE TINO WALTER

IPC: G01N35/10 ,  C12M1/22 ,  C12M1/26 ,  C12M1/36 ,  G01N1/04 ,  G01N35/00 ,  H01J49/16

CPC classification number: G01N35/00029 ,  C12M23/10 ,  C12M33/04 ,  C12M41/48 ,  C12Q3/00 ,  G01N35/10 ,  G01N2001/045 ,  G01N2035/1032 ,  H01J49/164

Abstract: A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.

Abstract translation: 一种用于在培养皿上定位和选择微生物菌落并使用MALDI鉴定所述选定菌落中的微生物的方法和装置。 该方法包括在培养皿上定位和选择微生物菌落的自动化步骤; 获得所述选定的微生物菌落的样品; 将所述选定的微生物菌落的所述样品中的至少一些沉积在靶板上; 以及在用于进行MALDI鉴定所述所选微生物菌落样品的设备中用所述样品转移所述靶板。 微生物菌落的样品自动沉积在沉积点上，使得样品至少覆盖目标板的所述一个沉积点的大约一半。 微生物样品的悬浮液通过用拾取工具自动采样样品并将采样工具用所述样品浸没在悬浮液中而自动制备，之后拾取工具在垂直方向上振动，以将样品从采摘工具中释放出来 。

公开(公告)号：WO2016191646A3

公开(公告)日：2017-01-26

申请号：PCT/US2016034554

申请日：2016-05-27

Applicant: BD KIESTRA B V ,  HANSEN TIMOTHY R ,  HOLTZ RICK ,  KLEEFSTRA MARTIJN ,  MARCELPOIL RAPHAEL RODOLPHE ,  PIERPONT RICK ,  POHL BRENT RONALD ,  SHEDLOSKY ALYSSA ,  SHINDLEDECKER SCOTT ,  SKEVINGTON EDWARD ,  SMITH KERRY LYNN ,  WILES TIMOTHY

Inventor： HANSEN TIMOTHY R ,  HOLTZ RICK ,  KLEEFSTRA MARTIJN ,  MARCELPOIL RAPHAEL RODOLPHE ,  PIERPONT RICK ,  POHL BRENT RONALD ,  SHEDLOSKY ALYSSA ,  SHINDLEDECKER SCOTT ,  SKEVINGTON EDWARD ,  SMITH KERRY LYNN ,  WILES TIMOTHY

IPC: G01N1/38 ,  G01N35/00 ,  G01N35/10

CPC classification number: G01N35/00029 ,  C12M33/04 ,  G01N1/38 ,  G01N35/0099 ,  G01N35/10 ,  G01N2035/00039 ,  G01N2035/00306 ,  G01N2035/1032

Abstract: A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provides for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.

Abstract translation: 一种用于在培养皿上定位和选择微生物菌落的方法和自动化装置，并使得到的样品进行多次下游测试，包括鉴定微生物的试验和用于鉴定微生物对抗生素敏感性的试验。 该方法包括在培养皿上定位和选择微生物菌落的自动化步骤; 获得所选择的微生物菌落的样品; 通过将采样工具与样品浸没在悬浮液中来自动制备微生物样品的悬浮液，然后拾取工具至少在垂直方向上振动，以将样品从悬浮液中的拾取工具中释放出来。 监测悬浮液的浊度，以确保悬浮液中的微生物浓度足以使悬浮液用于样品来源，用于样品中微生物的鉴定和抗生素敏感性。 该装置和系统任选提供用于抗生素敏感性试验（AST）制备的样品的下游处理。 这样的装置包括在用于AST测试的AST面板接种之后的进一步处理。 这种进一步的处理包括将接种的面板封盖并转移到AST仪器。