公开(公告)号：WO2017072078A1

公开(公告)日：2017-05-04

申请号：PCT/EP2016/075566

申请日：2016-10-24

Applicant: BRUDERER, Thomas ,  MURANJAN, Madhavi

Inventor： BRUDERER, Thomas ,  MURANJAN, Madhavi

IPC: G01N33/569 ,  C07K16/12

CPC classification number: G01N33/56938 ,  C07K16/00 ,  C07K16/1271

Abstract: A method for detecting the presence or absence of a first analyte and of a second analyte in a sample comprises a subtractive immunoassay including in the following order the steps of: a) conjugating a first detector antibody to the first analyte in order to tag the first analyte with a first detector tag; b) conjugating a first capture antibody to the first analyte in order to capture the first analyte and to completely deplete the first analyte from the sample; c) conjugating a second detector antibody to the second analyte in the depleted sample in order to tag the second analyte with a second detector tag; d) conjugating a second capture antibody to the second analyte in order to capture the second analyte from the sample; e) detecting the presence or absence of the first and second detector tag at the site of the first and second capture antibody, respectively, and thereby detecting the presence or absence of the first analyte and of the second analyte in the sample.

Abstract translation: 用于检测样品中第一分析物和第二分析物的存在或不存在的方法包括消减免疫测定法，其包括以下顺序的步骤：a）将第一检测抗体与 第一分析物，以便用第一检测器标签标记第一分析物; b）将第一捕获抗体缀合至第一分析物以捕获第一分析物并从样品中完全耗尽第一分析物; c）将第二检测抗体与耗尽样品中的第二分析物缀合以便用第二检测器标签标记第二分析物; d）将第二捕获抗体缀合至第二分析物以从样品中捕获第二分析物; e）分别在第一和第二捕获抗体的位点检测第一和第二检测器标签的存在或不存在，从而检测样品中第一分析物和第二分析物的存在或不存在。 >