公开(公告)号：WO1998006739A1

公开(公告)日：1998-02-19

申请号：PCT/US1997014487

申请日：1997-08-18

Applicant: CLONTECH LABORATORIES, INC.

Inventor： CLONTECH LABORATORIES, INC. ,  NELSON, Paul, S. ,  YANG, Te-Tuan ,  KAIN, Steven, R. ,  SMITH, Thomas, H.

IPC: C07K01/22

CPC classification number: B01D15/3828 ,  B01J20/3265 ,  B01J45/00 ,  C07K1/22 ,  C07K14/43595 ,  C07K2319/21 ,  C12N9/003 ,  G01N30/02 ,  B01D15/3804

Abstract: Purification of poly-amino acid-tagged recombinant proteins has been improved by the use of a carboxymethylated aspartate ligand complexed with a third-block transition metal having an oxidation state of 2 and a coordination number of 6. A method for synthesizing the metal ion-CM-Asp complex is also described. Further, the metal ion-CM-Asp complex can be used for screening protein function.

Abstract translation: 通过使用与氧化态为2 +和配位数为6的第三嵌段过渡金属络合的羧甲基化天冬氨酸配体，提高了多氨基酸标记的重组蛋白质的纯化。 还描述了金属离子-CM-Asp复合物。 此外，金属离子-CM-Asp复合物可用于筛选蛋白质功能。