公开(公告)号：WO2006134623A1

公开(公告)日：2006-12-21

申请号：PCT/IT2006/000449

申请日：2006-06-13

申请人： CONSIGLIO NAZIONALE DELLE RICERCHE ,  DEFEZ, Roberto

发明人： DEFEZ, Roberto

IPC分类号： A01C1/06 ,  A01N63/00 ,  C12N1/06 ,  C12N1/20 ,  C12P17/10

CPC分类号： C12R1/41 ,  C12N1/06 ,  C12N1/20 ,  C12P17/10

摘要： The present invention describes a method for increasing the survival of the bacteria of Rhizobium genus, comprising the steps of: making the bacteria to grow in a chemically defined medium; keeping the bacteria in growth stationary phase for a proper period of time; exposing the bacteria to effective quantities of indole-3 -acetic acid (IAA). Within the invention scope there is an alternative method to increase the survival of the bacteria of the Rhizobium genus by means of genetic engineering comprising the steps of: making a recombinant vector codifying enzymes able to produce IAA to express in effective way in said bacteria; making the bacteria to grow in chemically defined culture medium; keeping the bacteria in growth stationary phase for a proper period of time.

摘要翻译： 本发明描述了一种提高根瘤菌属细菌存活率的方法，包括以下步骤：使细菌在化学上确定的培养基中生长; 保持细菌生长稳定期一段适当的时间; 将细菌暴露于有效量的吲哚-3-乙酸（IAA）。 在本发明范围内，存在通过遗传工程增加根瘤菌属细菌存活的替代方法，包括以下步骤：制备能够产生IAA的重组载体，以有效地在所述细菌中表达; 使细菌在化学定义的培养基中生长; 保持细菌生长稳定期一段适当的时间。

公开(公告)号：WO2010109408A1

公开(公告)日：2010-09-30

申请号：PCT/IB2010/051249

申请日：2010-03-23

申请人： CONSIGLIO NAZIONALE DELLE RICERCHE ,  DEFEZ, Roberto

发明人： DEFEZ, Roberto

IPC分类号： C12N1/21 ,  C12R1/41

CPC分类号： C12P17/10 ,  C05F11/08 ,  C12P3/00

摘要： The present invention relates to the use of a bacterium having an high indole-3 -acetic acid (IAA) content for solubilizing phosphate rock (PR) in the ground, wherein said bacterium is obtained by transformation with a gene encoding an agent able to increase the IAA content.

摘要翻译： 本发明涉及具有高吲哚-3-乙酸（IAA）含量的细菌在地面中溶解磷酸盐岩（PR）的用途，其中所述细菌通过用编码能够增加的试剂的基因转化获得 IAA内容。