公开(公告)号：WO1989004675A1

公开(公告)日：1989-06-01

申请号：PCT/US1988003611

申请日：1988-10-17

Applicant: CREATIVE BIOMOLECULES, INC.

Inventor： CREATIVE BIOMOLECULES, INC. ,  HUSTON, James, S. ,  BAIRD, Lynn ,  COHEN, Charles ,  OPPERMAN, Hermann

IPC: A61M01/36

CPC classification number: A61M1/3679 ,  C07K14/31 ,  C07K17/06

Abstract: Disclosed is a method and a family of materials useful for removing immune complexes from blood preferentially to soluble antibodies. The material comprises analogs of proteins which bind to the Fc region of immunoglobulin. The analogs are produced by truncating or otherwise altering the amino acid sequence of the binding protein to reduce their affinity for Fc. An array of such analogs disposed about the surface of an insoluble matrix has the ability to form multiple points of attachment to the multiple Fc's in a complex so as to bind complex strongly, whereas only weak associations are developed between the Fc region of soluble IgG and individual analogs. The preferred analogs are truncated proteins homologous to a portion of the domains of Protein A or Protein G which bind with Fc. Complex may be removed from whole blood or serum using the material and conventional plasmapheresis techniques.

Abstract translation: 公开了一种用于从血液中将免疫复合物优先地去除可溶性抗体的方法和一系列材料。 该材料包含与免疫球蛋白Fc区结合的蛋白质的类似物。 通过截短或以其他方式改变结合蛋白的氨基酸序列来降低它们对Fc的亲和力来产生类似物。 在不溶性基质的表面附近设置的这种类似物的阵列具有在复合物中形成与多个Fc的多个连接点的能力，以便强烈地结合复合物，而仅在可溶性IgG的Fc区和 个别类似物。 优选的类似物是与蛋白A或蛋白G的结合部分与Fc结合的截短的蛋白质。 可以使用材料和常规的血浆置换技术从全血或血清中除去复合物。