公开(公告)号：WO2017144496A1

公开(公告)日：2017-08-31

申请号：PCT/EP2017/053986

申请日：2017-02-22

Applicant: DA VOLTERRA ,  BIOASTER

Inventor： DE GUNZBURG, Jean ,  DOCQUIER, Jean-Denis

IPC: C12N9/86

CPC classification number: C12N9/86 ,  A61K38/00 ,  C12Y305/02006 ,  A61K31/43 ,  A61K2300/00

Abstract: The present invention relates to an isolated polypeptide having beta-lactamase activity and nucleic acid sequences encoding the polypeptide. The isolated polypeptide of the invention is a VIM-2 variant with improved properties such as improved protease stability, stability in intestinal medium, improved activity against one or more antibiotics, improved specific activity and/or improved production in a host cell.

Abstract translation: 本发明涉及具有β-内酰胺酶活性的分离的多肽和编码该多肽的核酸序列。 本发明的分离的多肽是具有改进的性质如改进的蛋白酶稳定性，肠介质中的稳定性，改善的抗一种或多种抗生素的活性，提高的比活性和/或改善的宿主细胞生产的VIM-2变体。

公开(公告)号：WO2017144495A1

公开(公告)日：2017-08-31

申请号：PCT/EP2017/053985

申请日：2017-02-22

Applicant: DA VOLTERRA ,  BIOASTER

Inventor： DE GUNZBURG, Jean ,  DOCQUIER, Jean-Denis

IPC: C12N9/86

CPC classification number: C12N9/86

Abstract: The present invention relates to an isolated polypeptide having beta-lactamase activity and nucleic acid sequences encoding the polypeptide. The isolated polypeptide of the invention is a VIM-2 variant with improved properties such as improved protease stability.

Abstract translation: 本发明涉及具有β-内酰胺酶活性的分离的多肽和编码该多肽的核酸序列。 本发明的分离的多肽是具有改进的性质如改进的蛋白酶稳定性的VIM-2变体。

公开(公告)号：WO2023036886A1

公开(公告)日：2023-03-16

申请号：PCT/EP2022/075025

申请日：2022-09-08

Applicant: DA VOLTERRA

Inventor： DOCQUIER, Jean-Denis ,  DEVENTE, Savannah

IPC: C12N15/81 ,  A61K36/064 ,  A61P1/00 ,  C12N15/70 ,  C12N15/90

Abstract: The present invention relates to a yeast cell producing a functional antibiotic-inactivating enzyme. The yeast cell of the invention comprises an exogenous nucleic acid encoding the antibiotic-inactivating enzyme, wherein the exogenous nucleic acid comprises an intron able to prevent the production of a functional antibiotic-inactivating enzyme in a bacterial host cell.