公开(公告)号：WO9714797A2

公开(公告)日：1997-04-24

申请号：PCT/US9616782

申请日：1996-10-18

Applicant: DANA FARBER CANCER INST INC

Inventor： SOTIROPOULOU GEORGIA ,  ANISOWICZ ANTHONY ,  SAGER RUTH

IPC: A61K38/00 ,  A61K48/00 ,  C07K14/81 ,  C12N1/21 ,  C12N15/15 ,  A01K67/027 ,  A61K38/57 ,  A61K39/395 ,  C07K16/38 ,  C07K19/00 ,  C12N1/19 ,  C12N5/10 ,  C12N15/00 ,  C12N15/11 ,  C12N15/62 ,  C12Q1/68 ,  G01N33/68

CPC classification number: C07K14/8139 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00

Abstract: Isolated nucleic acid molecules encoding a novel cysteine proteinase inhibitor, cystatin M, which is a member of the Family 2 cystatins, are disclosed. Cystatin M exhibits cysteine proteinase inhibitory activity against papain, is downregulated in metastatic mammary epithelial tumor cells, as well as other tumor cells, and is upregulated in senescent cells. In addition to isolated nucleic acid molecules, the invention provides antisense nucleic acid molecules, recombinant expression vectors containing a nucelic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a cystatin M gene has been introduced or disrupted. The invention further provides isolated cystatin M proteins, fusion proteins, antigenic peptides and anti-cystatin M antibodies. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided.

Abstract translation: 公开了编码新型半胱氨酸蛋白酶抑制剂的分离的核酸分子，半胱氨酸蛋白酶抑制剂M是家族2胱氨酸蛋白酶的成员。 半胱氨酸蛋白酶抑制剂M表现出对木瓜蛋白酶的半胱氨酸蛋白酶抑制活性，在转移性乳腺上皮肿瘤细胞以及其他肿瘤细胞中下调，并在衰老细胞中上调。 除了分离的核酸分子之外，本发明提供了反义核酸分子，含有本发明的一种营养酸分子的重组表达载体，已经引入了表达载体的宿主细胞和其中半胱氨酸蛋白酶抑制剂M基因 已被引入或中断。 本发明还提供了分离的半胱氨酸蛋白酶抑制剂M蛋白，融合蛋白，抗原肽和抗半胱氨酸蛋白酶抑制剂M抗体。 还提供利用本发明组合物的诊断，筛选和治疗方法。

公开(公告)号：WO9811238A2

公开(公告)日：1998-03-19

申请号：PCT/US9716175

申请日：1997-09-11

Applicant: DANA FARBER CANCER INST INC

Inventor： ANISOWICZ ANTHONY ,  SAGER RUTH ,  SOTIROPOULOU GEORGIA

IPC: A61K38/00 ,  C12N9/64 ,  C12N15/57 ,  A01K67/027 ,  A61K31/70 ,  A61K38/48 ,  A61K39/395 ,  C07K16/40 ,  C12N5/10 ,  C12N15/00 ,  C12N15/11 ,  C12N15/62 ,  C12Q1/37 ,  C12Q1/68 ,  G01N33/574

CPC classification number: C12N9/6424 ,  A01K2217/05 ,  A61K38/00 ,  C07K2319/00

Abstract: Isolated nucleic acid molecules encoding a novel serine protease, Protease M, is disclosed. Protease M is downregulated in metastatic mammary epithelial tumor cells, as well as other tumor cells, and is upregulated in senescent cells. In addition to isolated nucleic acid molecules, the invention provides antisense nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a Protease M gene has been introduced or disrupted. The invention further provides isolated Protease M proteins, fusion proteins, antigenic peptides and anti-Protease M antibodies. Diagnostic assays, drug screening assays, and therapeutic methods utilizing compositions of the invention are also provided.

Abstract translation: 公开了编码新型丝氨酸蛋白酶蛋白酶M的分离的核酸分子。 蛋白酶M在转移性乳腺上皮肿瘤细胞以及其他肿瘤细胞中下调，并在衰老细胞中上调。 除了分离的核酸分子之外，本发明提供反义核酸分子，含有本发明的核酸分子的重组表达载体，已经引入了表达载体的宿主细胞和其中蛋白酶M基因的非人转基因动物 已被引入或中断。 本发明进一步提供分离的蛋白酶M蛋白，融合蛋白，抗原肽和抗蛋白酶M抗体。 还提供了使用本发明组合物的诊断测定，药物筛选测定和治疗方法。