公开(公告)号：WO2016149002A1

公开(公告)日：2016-09-22

申请号：PCT/US2016/021524

申请日：2016-03-09

申请人： ECOVATIVE DESIGN LLC

发明人： WINISKI, Jacob ,  VAN HOOK, Sue ,  LUCHT, Matthew ,  MCINTYRE, Gavin

IPC分类号： A01G1/04 ,  C12N1/14

CPC分类号： C12N1/14 ,  C12N1/22

摘要： In one embodiment, the process combines a fraction (up to 15%) of a lignocellulose substrate with supplemental nutritional material and hydrates the fraction to a moisture content of from 40% to 70% by weight. The hydrated substrate fraction is heat processed to remove ambient bioburden (yeast, mold, bacteria) and to maintain the hydrated substrate fraction in an aseptic condition. Thereafter, the hydrated substrate fraction is inoculated with a fungus and incubated to obtain a myceliated substrate which is then reduced into discrete particles. The remaining fraction of the substrate is combined with water and then combined with the discrete particles of myceliated substrate and incubated to obtain a second myceliated substrate which is then reduced into discrete particles. The second myceliated substrate is combined with supplemental nutritional material and incubated to obtain a third myceliated substrate composed of at least 10% mycelium.

摘要翻译： 在一个实施方案中，该方法将木质纤维素基质的一部分（至多15％）与补充营养材料相结合，并将该部分水合成水分含量为40重量％至70重量％。 将水合的底物部分热处理以除去环境中的生物负载（酵母，霉菌，细菌）并将水合的底物部分保持在无菌状态。 此后，用真菌接种水合的底物部分，并孵育以获得一种骨髓基质，然后将其还原成离散的颗粒。 将底物的剩余部分与水混合，然后与离心的底物的离散颗粒组合，并孵育以获得第二个胶原底物，然后将其还原成离散的颗粒。 将第二个含有骨髓的底物与补充的营养物质组合，并孵育以获得由至少10％的菌丝体组成的第三个myceliate底物。