公开(公告)号：WO0208454A3

公开(公告)日：2002-06-27

申请号：PCT/US0123358

申请日：2001-07-24

Applicant: GENPRIME INC ,  FLEMING JAMES E ,  SOMES JASON BUCK ,  MCLEAN DARBY ,  HOLCOMB JERAD R

Inventor： FLEMING JAMES E ,  SOMES JASON BUCK ,  MCLEAN DARBY ,  HOLCOMB JERAD R

IPC: C12Q1/06

CPC classification number: C12Q1/06 ,  Y10T436/101666

Abstract: A rapid method for the quantitation of various live cell types is described. The method may include a variety of steps including: 1) suspending the cells in a detergent-like compound; 2) isolating the washed cells by centrifugation or filtration; 3) resuspending the cells in a solution that contains a preservative, a fluorescent dye and a compound such as dequalinium which can be taken up by the cells; 4) measuring the fluorescence increase over time of the cell-dye mixture with a simple fluorometer; and 5) measuring the native fluorescence of the cells. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods; b) quantitating yeast cells in brewing, fermentation and bread making; c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The device, and/or system used for cell quantitation is also disclosed.

Abstract translation: 描述了用于定量各种活细胞类型的快速方法。 该方法可以包括多种步骤，包括：1）将细胞悬浮在洗涤剂状化合物中; 2）通过离心或过滤分离洗涤的细胞; 3）将细胞重悬于包含防腐剂，荧光染料和可被细胞吸收的脱金属化合物的溶液中; 4）用简单的荧光计测量细胞染料混合物随时间的荧光增加; 和5）测量细胞的天然荧光。 这种新的细胞荧光方法与其他枚举细胞的方法相关，例如标准平板计数，亚甲基蓝法和载玻片活力技术。 该方法在几个应用中特别有用，例如：a）定量分析牛奶，酸奶，奶酪，肉类和其他食物中的细菌; b）定量酿造，发酵和面包制作中的酵母细胞; c）在研究，食物和临床环境中定量哺乳动物细胞。 当需要总细胞计数和活细胞计数时，如在酿造工业中，该方法特别有用。 该方法也可用于确定样品中细胞的代谢活性。 还公开了用于细胞定量的装置和/或系统。

公开(公告)号：WO0136661A3

公开(公告)日：2002-07-11

申请号：PCT/US0041545

申请日：2000-10-24

Applicant: GENPRIME INC ,  FLEMING JAMES E ,  SOMES JASON BUCK

Inventor： FLEMING JAMES E ,  SOMES JASON BUCK

IPC: C12Q1/04 ,  G01N33/50 ,  C12Q1/06

CPC classification number: G01N33/5094 ,  C12Q1/04 ,  G01N33/5005

Abstract: This invention describes methods and kits for quantifying viable cells in a sample using fluorescent dyes that can be internalized predominately by viable cells and have fluorescence properties measurably altered when bound to target components. These methods circumvent the need for training personnel in plating, growing and count viable cells, and reduce both the time and the cost required for cellular quantitation that use existing methods.

Abstract translation: 本发明描述了使用荧光染料定量样品中的活细胞的方法和试剂盒，其可以主要由活细胞内化，并且当与靶组分结合时具有可测量改变的荧光性质。 这些方法规避了对电镀，生长和计数活细胞培养人员的需求，并减少使用现有方法的细胞定量所需的时间和成本。

公开(公告)号：WO2010021873A3

公开(公告)日：2010-06-24

申请号：PCT/US2009053404

申请日：2009-08-11

Applicant: GENPRIME INC ,  FLEMING JAMES E ,  MCGREW JESSE M ,  MCGREW STEPHEN P ,  NICHOLES PATRICK ,  HUMPHREYS JOHNNY

Inventor： FLEMING JAMES E ,  MCGREW JESSE M ,  MCGREW STEPHEN P ,  NICHOLES PATRICK ,  HUMPHREYS JOHNNY

IPC: G01N35/00 ,  G01N30/74 ,  G06F19/00

CPC classification number: G01N21/77 ,  G01N21/8483 ,  G01N2035/00108 ,  G16H10/40 ,  G16H40/63

Abstract: An assay system includes an optical imager to acquire high resolution images of assay strips (e.g., lateral flow immunochromatographic test strips) and performs image processing to identify individual assay strips and determine results for each assay strip, by quantifies the presence or absence of test signal line(s) and control signal line(s). Assay strips may be in a holder or carrier contained in a specimen container also holding a specimen. The assay system automatically logs all results and data to a database that stores a high resolution image of the original immunochromatographic assay, the values of test line(s) and control line(s), and the test result. A user interface directs an end user through operation.

Abstract translation: 测定系统包括光学成像仪以获得测定条的高分辨率图像（例如横向流动免疫层析测试条），并执行图像处理以识别各个测定条，并通过量化测试信号的存在或不存在来确定每个测定条的结果 线路和控制信号线。 测定条可以在包含在也保持标本的样本容器中的保持器或载体中。 测定系统将所有结果和数据自动记录到存储原始免疫色谱测定的高分辨率图像，测试线和对照线的值以及测试结果的数据库。 用户界面指示最终用户通过操作。