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    NOVEL STEROID 5 α -REDUCTASE
    1.
    发明申请
    NOVEL STEROID 5 α -REDUCTASE 审中-公开
    NOVEL STEROID 5 a -REDUCTASE

    公开(公告)号：WO2006122214A2

    公开(公告)日：2006-11-16

    申请号：PCT/US2006/018147

    申请日：2006-05-10

    申请人： HEALTH RESEARCH INC. ,  THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL ,  MOHLER, James, L. ,  TITUS, Mark ,  KOZYREVA, Olga ,  FORD, O'Harris,III ,  KAWINSKI, Elsbieta ,  LI, Yun

    发明人： MOHLER, James, L. ,  TITUS, Mark ,  KOZYREVA, Olga ,  FORD, O'Harris,III ,  KAWINSKI, Elsbieta ,  LI, Yun

    IPC分类号： C12Q1/68 ,  G01N33/574 ,  C12N9/02 ,  C12N15/861

    CPC分类号： C12Q1/26 ,  C07K2319/21 ,  C07K2319/40 ,  C12N9/001 ,  C12N2799/022 ,  G01N33/57434

    摘要： The invention provides an isolated, novel steroid 5α-reductase enzyme termed SRD5AIIL The protein has an estimated molecular weight of 37 kDa and is capable of converting testosterone to dihydrotestosterone at a pH of about 7.0. Also provided is a method for identifying inhibitors of SRD5 AIII by contacting SRD5 AIII with a test compound and measuring the activity of the enzyme. A reduced activity relative to a control indicates that the test compound is an inhibitor of SRD5AIII. A method is also provided for detecting androgen stimulated prostate cancer or recurrent prostate cancer in an individual. The method comprises obtaining a prostate biopsy from an individual and determining the level of expression of SRD5ADI gene or protein relative to a normal control. An increased expression of SRD5AIII relative to the control is indicative of androgen stimulated prostate cancer or recurrent prostate cancer.

    摘要翻译： 本发明提供了一种称为SRD5AIIL的分离的新型类固醇5a-还原酶。该蛋白质具有37kDa的估计分子量，并且能够在约7.0的pH下将睾酮转化为二氢睾酮。 还提供了通过将SRD5AIII与测试化合物接触并测量酶的活性来鉴定SRD5AIII的抑制剂的方法。 相对于对照的降低的活性表明测试化合物是SRD5AIII的抑制剂。 还提供了用于检测个体中激素刺激的前列腺癌或复发性前列腺癌的方法。 该方法包括从个体获得前列腺活检，并确定SRD5ADI基因或蛋白质相对于正常对照的表达水平。 SRD5AIII相对于对照的增加的表达指示雄激素刺激的前列腺癌或复发性前列腺癌。

    INCONTINENCE DEVICE
    2.
    发明申请
    INCONTINENCE DEVICE 审中-公开

    公开(公告)号：WO2019084511A1

    公开(公告)日：2019-05-02

    申请号：PCT/US2018/057871

    申请日：2018-10-26

    申请人： HEALTH RESEARCH, INC.

    发明人： LEWCZYK, Christopher ,  MOHLER, James, L.

    IPC分类号： A61F13/47 ,  A61F13/56 ,  A61F13/15

    CPC分类号： A61F13/47 ,  A61F13/5605

    摘要： The present disclosure may be embodied as an incontinence device for a catheterized individual. The device includes an absorbent pad configured to be worn by the individual. The absorbent pad having a slit partially separating the pad into two portions. A fastener may be provided for selectively attaching the two portions to each other. In this way, a catheter may be disposed through the slit of the device and the two portions may be attached to each other using the fastener to surround a circumference of the catheter. The fastener may be reusable. In some embodiments, the device includes more than one fastener. The fastener may be one or more of an adhesive, a hook-and-loop fastener, a snap fastener, or combinations thereof.

    METHOD FOR DETERMINATION OF DHT LEVELS IN TISSUE SAMPLES
    4.
    发明申请
    METHOD FOR DETERMINATION OF DHT LEVELS IN TISSUE SAMPLES 审中-公开
    用于确定组织样品中DHT水平的方法

    公开(公告)号：WO2008045323A2

    公开(公告)日：2008-04-17

    申请号：PCT/US2007/021399

    申请日：2007-10-08

    申请人： HEALTH RESEARCH INC. ,  THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES ,  MOHLER, James, L. ,  TITUS, Mark ,  TOMER, Kenneth

    发明人： MOHLER, James, L. ,  TITUS, Mark ,  TOMER, Kenneth

    IPC分类号： G01N33/92

    CPC分类号： G01N33/743 ,  G01N30/724

    摘要： Provided is a method for determining the presence or amount of 5-α-dihydro-3-keto steroids (ADKSs) comprising the steps of preparing a sample for chromatographic separation, subjecting the sample to chromatographic separation, subjecting the chromatographically separated sample to atmospheric pressure photoionization (APPI), and detecting the amount of alkylated parent ions using mass spectrometry or methylated daughter ions by mass spectrometry after collisionally induced dissociation of the alkylated parent ions. Tandem mass spectrometry of ADKSs with a 6-member A ring and a 3-keto group using APPI and methanol as the alkoxy donor will produce methylated daughter ions with a mass/charge ratio of 85.0. Quantitation of this alkylated daughter ion provides a method to detect very low levels of ADKSs such as DHT. This method can also be used for the simultaneous detection of ADKSs and non-ADKSs.

    摘要翻译： 提供了一种用于测定5-a-二氢-3-酮类固醇（ADKS）的存在或量的方法，包括制备用于色谱分离的样品的步骤，对样品进行色谱分离，使色谱分离的样品经受大气压 光电离（APPI），并且在碰撞诱导的烷基化母体离子解离之后通过质谱法检测使用质谱法或甲基化子离子的烷基化亲本离子的量。 使用APPI和甲醇作为烷氧基供体的具有6个成员的A环和3个酮基的ADKS的串联质谱法将产生质量/电荷比为85.0的甲基化的子离子。 这种烷基化的子离子的定量提供了检测非常低水平的ADKS如DHT的方法。 该方法也可用于同时检测ADKS和非ADKS。