公开(公告)号：WO2023062542A2

公开(公告)日：2023-04-20

申请号：PCT/IB2022/059754

申请日：2022-10-11

Applicant: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC

Inventor： ARGYROS, Aaron ,  VAN DIJKEN, Johannes Pieter ,  CARIGNAN, Bailey Morgan ,  BARRETT, Trisha ,  STONEHOUSE, Emily

IPC: C07K14/395 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12P7/06

Abstract: The present disclosure provides a recombinant yeast cell for making ethanol. The recombinant yeast cell comprises a first genetic modification to increase an ethanol yield in the recombinant yeast cell when compared to a parental yeast cell. The recombinant yeast cell also comprises a second genetic modification capable of increasing pyruvate decarboxylase activity in the recombinant yeast cell when compared to the parental yeast cell. The parental yeast cell lacks the first genetic modification and the second genetic modification. The present disclosure also provides methods for making the recombinant yeast cell as well processes for using the recombinant yeast cell to make ethanol.

公开(公告)号：WO2015023989A1

公开(公告)日：2015-02-19

申请号：PCT/US2014/051355

申请日：2014-08-15

Applicant: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC

Inventor： ARGYROS, Aaron ,  KENEALY, William R. ,  STONEHOUSE, Emily

IPC: C12N1/22 ,  C12N9/24 ,  C12N15/81

CPC classification number: C12P7/06 ,  C07K14/395 ,  C12N1/22 ,  C12N1/32 ,  C12N9/0006 ,  C12N15/81 ,  C12P7/04 ,  C12Y101/01008 ,  Y02E50/17

Abstract: The present invention provides for novel metabolic pathways to reduce or modulate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous proteins that function to import glycerol and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous proteins or enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more native or heterologous proteins that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous proteins or enzymes is activated, upregulated or downregulated. Also provided are methods for increasing cellular glycerol uptake and increasing recombinant production of fuels and other chemicals using the recombinant microorganisms of the invention.

Abstract translation: 本发明提供用于减少或调节甘油产生并增加产物形成的新型代谢途径。 更具体地，本发明提供了包含一种或多种天然和/或异源蛋白质的重组微生物，所述蛋白质用于导入甘油和一种或多种在一个或多个工程化代谢途径中起作用以转化碳水化合物源的天然和/或异源酶， 例如木质纤维素，产物如乙醇，其中一种或多种天然和/或异源蛋白质或酶被激活，上调或下调。 本发明还提供了一种重组微生物，其包含一种或多种用于调节甘油合成的天然或异源蛋白质和一种或多种在一个或多个工程化代谢途径中起作用以将碳水化合物源转化为乙醇的天然和/或异源酶，其中 所述一种或多种天然和/或异源蛋白质或酶被激活，上调或下调。 还提供了使用本发明的重组微生物增加细胞甘油摄取和增加燃料和其它化学品的重组生产的方法。