公开(公告)号：WO2015040497A3

公开(公告)日：2015-07-23

申请号：PCT/IB2014002816

申请日：2014-09-22

Applicant: LONZA AG

Inventor： WALSH PATRICK ,  FELLNER THOMAS

IPC: C12N5/0789

CPC classification number: C12N5/0696 ,  A61K33/08 ,  C12N2500/12 ,  C12N2500/90 ,  C12N2506/11 ,  C12N2506/115 ,  C12N2533/52

Abstract: Described herein are methods for enhancing the nuclear reprogramming of somatic cells to become induced pluripotent stem cells. In particular, the methods disclosed herein involve the use of damage-associated molecular pattern molecules (DAMP). In certain embodiments the DAMPs are aluminum compositions such as aluminum hydroxide. Such DAMPs have unexpectedly and surprisingly been found to enhance the nuclear reprogramming efficiency of the reprogramming factors commonly used to induce somatic cells to become induced pluripotent stem cells. Accordingly, this disclosure describes methods of nuclear reprogramming as well as cells obtained from such methods along with therapeutic methods for using such cells for the treatment of disease amendable to treatment by stem cell therapy; as well as kits for such uses.

Abstract translation: 本文描述了用于增强体细胞核重编程以成为诱导多能干细胞的方法。 特别地，本文公开的方法涉及使用损伤相关分子模式分子（DAMP）。 在某些实施方案中，DAMP是铝组合物，例如氢氧化铝。 已经意外地惊奇地发现了这种DAMP以提高通常用于诱导体细胞成为诱导多能干细胞的重编程因子的核重编程效率。 因此，本公开描述了核重新编程的方法以及从这些方法获得的细胞以及使用这种细胞治疗可通过干细胞治疗治疗的疾病的治疗方法; 以及用于此类用途的套件。