公开(公告)号：WO2019113050A1

公开(公告)日：2019-06-13

申请号：PCT/US2018/063822

申请日：2018-12-04

Applicant: LONZA LTD ,  LONZA INC.

Inventor： WILKES, Benjamin, Alexander

IPC: G01N33/64 ,  C07C49/717

CPC classification number: G01N33/64 ,  C07C45/79 ,  C07C49/717

Abstract: Disclosed herein are methods useful for detecting and/or quantifying tropolone in samples, e.g., generated during the production of and in final formulations of a product, e.g., a recombinant protein, e.g., an antibody. Tropolone and derivatives thereof (cycloheptatriene ketones) are separated from a mixture by adding a partially or fully fluorinated alkyl or aryl (e.g. pentafluorophenylpropyl), which (covalently) binds the tropolone-like compound. Then it is assayed via UV or tandem mass spectrometry. A reaction mixture of tropolone-like compounds and fluorinated alkyl or aryl is also disclosed.