公开(公告)号：WO2013181615A1

公开(公告)日：2013-12-05

申请号：PCT/US2013/043732

申请日：2013-05-31

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： BIRCH, Christina, Marie ,  HOU, Han, Wei ,  HAN, Jongyoon ,  NILES, Jacquin, C.

IPC: C12M3/06

CPC classification number: C12N15/1048 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  C12N15/1003

Abstract: A method of separating one or more binding molecules that are specifically bound to one or more binding targets from a mixture of bound and unbound molecules includes introducing the mixture into at least one inlet of a microfluidic device at a sample flow rate. The microfluidic device includes at least one curved channel, wherein each curved channel has a length, a radius, and a cross-section of a height and a width defining an aspect ratio adapted to isolate the one or more molecules bound to the binding target along portions of the cross-section of the channel based on size. Bound molecules flow along a radially innermost portion of the channel to a first outlet, and unbound molecules flow along one or more other portions of the channel to at least one other outlet, thereby separating the one or more binding molecules that are specifically bound to the one or more binding targets from the mixture of bound and unbound molecules.

Abstract translation: 分离从结合和未结合分子的混合物特异性结合一个或多个结合靶的一个或多个结合分子的方法包括以样品流速将混合物引入至少一个微流体装置的入口。 微流体装置包括至少一个弯曲通道，其中每个弯曲通道具有长度，半径和横截面，其高度和宽度限定了适于隔离结合到结合靶的一个或多个分子的纵横比 基于尺寸的通道的横截面的部分。 结合的分子沿着通道的径向最内部分流动到第一出口，未结合的分子沿着通道的一个或多个其它部分流动到至少一个其它出口，从而将一个或多个特异性结合到 来自结合和未结合分子的混合物的一种或多种结合靶。