公开(公告)号：WO2019170625A1

公开(公告)日：2019-09-12

申请号：PCT/EP2019/055359

申请日：2019-03-05

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E. V.

Inventor： KUPKE, Sascha ,  FRENSING, Timo ,  ZMORA, Pawel ,  REICHL, Udo

IPC: C12N7/00

Abstract: In a first aspect, the present invention relates to an isolated nucleic acid molecule, in particular, an RNA molecule containing particular substitutions. In a further aspect, the present invention relates to a composition comprising the same as well as virus-like particle, viral vector or virus particle containing the nucleic acid molecule according to the present invention. The virus-like particle, the viral vector or the virus particle is suitable for use as a medicament in particular for treating viral infection. Further, a pharmaceutical composition is provided in particular suitable for use as a pharmaceutical prophylactic vaccine containing the virus-like particle, viral vector or the virus particle or the nucleic acid molecule according to the present invention. Finally, a vaccine for vaccination against viral infection, in particular, influenza viral infection is provided as well as a composition or kit comprising the virus-like particle, viral vector or the virus particle according to the present invention or the nucleic acid molecule according to the present invention in a combination with a wild type viral vector or wild type virus particle said composition or kit is suitable for use in treating or protecting against viral infection.

公开(公告)号：WO2016005363A1

公开(公告)日：2016-01-14

申请号：PCT/EP2015/065433

申请日：2015-07-07

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E. V.

Inventor： WOLFF, Michael ,  PIELER, Michael Martin ,  REICHL, Udo

IPC: C08B5/14 ,  C12N7/00

CPC classification number: C08B5/14 ,  C12N7/00 ,  C12N2760/16151

Abstract: In a first aspect, the present invention relates to a process for the preparation of magnetic sulfated cellulose particles. In particular, the present invention relates to the preparation of magnetic sulfated cellulose particles under specific reaction conditions allowing providing said magnetic sulfated cellulose particles being particularly useful in further process steps including enrichment of proteinaceous compositions. In another aspect, the present invention relates to the magnetic sulfated cellulose particles itself as well as its use for isolation of proteinaceous compositions including composition of virus proteins and/or non-virus proteins as well as whole virus and for enrichment steps in analytical methods. Finally, the present invention provides a method for isolating whole virus or virus proteins as well as isolating heparin binding molecules comprising the step of pseudo-affinity purification using the magnetic sulfated cellulose particles according to the present invention. The present invention relates further to a bioreactor system comprising a bioreactor and a magnetic separation unit useful for separating magnetic sulfated cellulose particles according to the present invention.

Abstract translation: 在第一方面，本发明涉及制备磁性硫酸化纤维素颗粒的方法。 特别地，本发明涉及在特定反应条件下制备磁性硫酸化纤维素颗粒，使得所述磁性硫酸化纤维素颗粒特别可用于进一步的工艺步骤，包括富集蛋白质组合物。 另一方面，本发明涉及磁性硫酸化纤维素颗粒本身以及其用于分离蛋白质组合物的用途，所述蛋白质组合物包括病毒蛋白质和/或非病毒蛋白质的组合以及全病毒并用于分析方法中的富集步骤。 最后，本发明提供一种用于分离整个病毒或病毒蛋白质以及分离肝素结合分子的方法，其包括使用根据本发明的磁性硫酸化纤维素颗粒的假亲和纯化步骤。 本发明进一步涉及包含生物反应器和用于分离本发明的磁性硫酸化纤维素颗粒的磁分离单元的生物反应器系统。