公开(公告)号：WO2018226828A3

公开(公告)日：2018-12-13

申请号：PCT/US2018/036256

申请日：2018-06-06

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： WOO, Christina, M. ,  GAO, Jinxu ,  AMAKO, Yuka ,  FU CHANG, Chia ,  LIN, Zhi

IPC: C07K1/13 ,  C07D229/02 ,  G01N33/58

Abstract: Provided herein are methods, systems, kits, and compositions useful for determining small molecule-protein interactions and protein-protein interactions. The photo-click tags provided herein can be conjugated to a small molecule or amino acid analog to provide compounds that can be integrated into a protein through photo-conjugation, allowing for identification of a small molecule-protein interaction or protein-protein interaction to elucidate the small molecules mechanism of action or the protein targeted by the small molecule. In some embodiments, the photo-click tags comprise a photo-conjugation moiety and a click chemistry handle, allowing for the attachment of various functional groups (e.g., affinity tags) to the small molecule or amino acid analog.

公开(公告)号：WO2018226828A2

公开(公告)日：2018-12-13

申请号：PCT/US2018/036256

申请日：2018-06-06

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： WOO, Christina, M. ,  GAO, Jinxu

IPC: C07D229/02 ,  A61K31/396

Abstract: Provided herein are methods, systems, kits, and compositions useful for determining small molecule-protein interactions and protein-protein interactions. The photo-click tags provided herein can be conjugated to a small molecule or amino acid analog to provide compounds that can be integrated into a protein through photo-conjugation, allowing for identification of a small molecule-protein interaction or protein-protein interaction to elucidate the small molecules mechanism of action or the protein targeted by the small molecule. In some embodiments, the photo-click tags comprise a photo-conjugation moiety and a click chemistry handle, allowing for the attachment of various functional groups (e.g., affinity tags) to the small molecule or amino acid analog.