公开(公告)号：WO2021061790A1

公开(公告)日：2021-04-01

申请号：PCT/US2020/052243

申请日：2020-09-23

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： STAIRS, Robert ,  REILLY, James ,  MATTILA, John ,  WADSWORTH, Samantha

IPC: G01N30/40 ,  G01N30/50

Abstract: Aspects of the present disclosure relate to a method of regenerating a hydrophobic interaction chromatography column to which a load mass has been applied, the method comprising passing one or more column volumes of an alkaline solution through hydrophobic interaction media within the column, wherein the alkaline solution exhibits a pH of between about 10 and about 14, and a conductivity of between 0.5 mS/cm and about 10 mS/cm, wherein material bound to the hydrophobic interaction media is removed. In some cases, the alkaline solution may include sodium hydroxide at a concentration of between, e.g., about 0.1 mM and 10 mM.