公开(公告)号：WO2022175592A1

公开(公告)日：2022-08-25

申请号：PCT/FI2022/050090

申请日：2022-02-15

Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT OY

Inventor： KOIVURANTA, Kari ,  BLOMSTER ANDBERG, Martina ,  NYGREN, Heli ,  CASTILLO, Sandra

IPC: C12N9/14 ,  C08J11/10 ,  C12N15/00

Abstract: The present invention relates to the fields of life sciences, micro-organisms and degradation of hydrocarbon chains such as plastics or synthetic polymers. Specifi-cally, the invention relates to an isolated specific enzyme, or a fragment thereof, wherein said enzyme or fragment is capable of degrading a hydrocarbon chain, and to a micro-organism or a host cell comprising the enzyme or a fragment thereof. Also, the present invention relates to a polynucleotide encoding the enzyme or fragment thereof, and to an expression vector or plasmid comprising the polynucleotide of the present invention. And still, the present invention relates to use of the enzyme, fragment, micro-organism, host cell, polynucleotide, expression vector or plasmid of the present invention for degrading a hydrocarbon chain; to a method of degrading a hydrocarbon chain with the specific enzyme or a fragment thereof; and to a method of producing the enzyme or fragment thereof of the present invention.

公开(公告)号：WO2023285738A2

公开(公告)日：2023-01-19

申请号：PCT/FI2022/050495

申请日：2022-07-12

Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT OY

Inventor： KOIVURANTA, Kari ,  CASTILLO, Sandra ,  NYGREN, Heli

IPC: C12P7/625 ,  C12P5/02 ,  C12P7/04 ,  C12P7/24 ,  C12P7/40 ,  C12N9/02 ,  C12N9/08 ,  B09B3/60 ,  C08J11/10 ,  C12R1/10 ,  C12R1/085 ,  C12R1/125 ,  C08J11/105 ,  C08J2323/02 ,  C12N9/001 ,  C12Y111/0101 ,  C12Y115/01001

Abstract: The present invention relates to the fields of life sciences, micro- organisms and degradation of hydrocarbon chains such as polyolefins. Specifically, the invention relates to an isolated specific enzyme or a fragment thereof, wherein said enzyme or fragment is a superoxide dismutase and is capable of degrading a hydrocarbon chain or a polyolefin, and to a micro-organism or a host cell comprising the enzyme or a fragment thereof. Also, the present invention relates to a polynucleotide encoding the enzyme or fragment thereof, and to an expression vector or plasmid comprising the polynucleotide of the present invention. And still, the present invention relates to use of the enzyme, fragment, micro-organism, host cell, polynucleotide, expression vector or plasmid of the present invention for degrading a hydrocarbon chain such as a polyolefin; to a method of degrading a hydrocarbon chain such as a polyolefin with the specific superoxide dismutase enzyme or a fragment thereof; and to a method of producing the enzyme or fragment thereof of the present invention.

公开(公告)号：WO2022219239A2

公开(公告)日：2022-10-20

申请号：PCT/FI2022/050238

申请日：2022-04-12

Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT OY

Inventor： KOIVURANTA, Kari ,  TSITKO, Irina ,  CASTILLO, Sandra ,  NYGREN, Heli

IPC: C12N9/08 ,  B09B3/60 ,  C08J11/10 ,  C12P7/00 ,  C08J11/105 ,  C08J2323/00 ,  C12N9/0065

Abstract: The present invention relates to the fields of life sciences, micro-organisms and degradation of polyolefin polymers. Specifically, the invention relates to an isolated specific enzyme or a fragment thereof, wherein said enzyme or fragment is capable of degrading a polyolefin, and to a micro-organism or a host cell comprising the enzyme or a fragment thereof. Also, the present invention relates to a polynucleotide encoding the enzyme or fragment thereof, and to an expression vector or plasmid comprising the polynucleotide of the present invention. And still, the present invention relates to use of the enzyme, fragment, micro-organism, host cell, polynucleotide, expression vector or plasmid of the present invention for degrading a polyolefin; to a method of degrading a polyolefin with the specific enzyme or a fragment thereof; and to a method of producing the enzyme or fragment thereof of the present invention.

公开(公告)号：WO2020157381A1

公开(公告)日：2020-08-06

申请号：PCT/FI2020/050042

申请日：2020-01-28

Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT OY

Inventor： TULLILA, Antti ,  NEVANEN, Tarja ,  AROLA, Henri ,  NYGREN, Heli

IPC: G01N33/53 ,  G01N33/543

Abstract: The present invention relates to the fields of life sciences, polypeptide analysis and polypeptide discovery methods. Also, the present invention concerns detection methods of target molecules in a sample. Specifically, the invention relates to a method of screening polypeptides capable of binding a target molecule and a method of analyzing or pretreating a sample. Also, the present invention relates to a kit for carrying out methods of the present invention as well as to a polypeptide, antibody or antigen binding fragment capable of binding target molecules screened or obtained by the method of the present invention.