公开(公告)号：WO1998018157A1

公开(公告)日：1998-04-30

申请号：PCT/US1997018076

申请日：1997-10-01

Applicant: THE UNIVERSITY OF WASHINGTON

Inventor： THE UNIVERSITY OF WASHINGTON ,  HOLL, Mark, R. ,  SABETI, Roya, R.

IPC: H01L21/00

CPC classification number: H01L21/68

Abstract: This invention provides rapid, accurate and repeatable alignment of components in three spatial coordinate directions to within about +/-10 mu m. It comprises a two-stage kinematic mount and a corresponding two-stage mountable module, each having a macrostage for initial alignment and a microstage for further, high accuracy alignment. The microstage of the mount preferably has either three alignment pins or three constraints and the microstage of the mountable module likewise preferably has either three pins or three constraints, so that the constraints of one component receive the pins of the other. A compliant layer is positioned between the microstage and macrostage of the mountable module. This invention further includes two-stage mount and mountable modules incorporating optical, electrical, mechanical or fluidic elements. In a preferred embodiment, the microstage module has a V-groove fluid flow channel suitable for use in optical flow cytometry.

Abstract translation: 本发明提供了在三个空间坐标方向上的组件的快速，准确和可重复的对准，在约+/-10μm内。 它包括两级运动安装座和相应的两级可安装模块，每个模块具有用于初始对准的宏观阶段和用于进一步高精度对准的微型台阶。 安装架的微型台阶优选地具有三个对准销或三个约束，并且可安装模块的微步骤同样优选地具有三个引脚或三个约束，使得一个部件的约束接收另一个部件的引脚。 柔性层位于可安装模块的微台阶和宏观阶段之间。 本发明还包括并入光学，电气，机械或流体元件的两级安装和安装模块。 在优选的实施方案中，微步骤模块具有适合用于光流式细胞术的V沟流体流动通道。

公开(公告)号：WO1997000442A1

公开(公告)日：1997-01-03

申请号：PCT/US1996010308

申请日：1996-06-14

Applicant: THE UNIVERSITY OF WASHINGTON

Inventor： THE UNIVERSITY OF WASHINGTON ,  YAGER, Paul ,  BRODY, James, P. ,  HOLL, Mark, R. ,  FORSTER, Fred, K. ,  GALAMBOS, Paul, C.

IPC: G01N30/00

CPC classification number: B01D11/0492 ,  B01D11/0496 ,  B01F13/0059 ,  B01F13/0093 ,  B01F2005/0031 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00889 ,  B01J2219/00905 ,  B01J2219/00952 ,  B01J2219/0097 ,  B01J2219/00995 ,  G01N15/06 ,  G01N30/0005 ,  G01N2001/4072 ,  G01N2035/00237 ,  Y10S366/01 ,  Y10T436/255

Abstract: This invention provides a microfabricated extraction system and methods for extracting desired particles from a sample stream containing desired and undesired particles. The sample stream is placed in laminar flow contact with an extraction stream under conditions in which inertial effects are negligible. The contact between the two streams is maintained for a sufficient period of time to allow differential transport of the desired particles from the sample stream into the extraction stream. In a preferred embodiment the differential transport mechanism is diffusion. The extraction system of this invention coupled to a microfabricated diffusion-based mixing device and/or sensing means allows picoliter quantities of fluid to be processed or analyzed on devices no larger than silicon wafers.

Abstract translation: 本发明提供了一种微加工提取系统和从含有所需和不需要的颗粒的样品流中提取所需颗粒的方法。 在惯性效应可忽略的条件下，将样品流与提取流层流接触。 保持两个物流之间的接触足够的时间，以便将期望的颗粒从样品流中差分输送到萃取物流中。 在优选实施例中，差分传送机构是扩散。 本发明的提取系统耦合到基于微制造的基于扩散的混合装置和/或感测装置允许在不大于硅晶片的装置上处理或分析皮升数量的流体。