公开(公告)号：WO2012058513A1

公开(公告)日：2012-05-03

申请号：PCT/US2011/058226

申请日：2011-10-28

Applicant: THERMO FINNIGAN LLC ,  BRANN, John, E. ,  FRANCE, Eric, A.

Inventor： BRANN, John, E. ,  FRANCE, Eric, A.

IPC: G01N30/00 ,  G01N30/60

CPC classification number: B01L3/563 ,  B01D15/22 ,  B01L2300/041 ,  B23P19/04 ,  F16L37/20 ,  G01N30/461 ,  G01N30/60 ,  G01N30/6039 ,  Y10T29/49904

Abstract: An apparatus for coupling a liquid chromatography column comprising an end fitting to a tubing comprises: at least one body member comprising at least one chamber; a first spring within the at least one chamber, the first spring configured so as to apply a first force to the tubing towards the column end fitting; a second spring within the at least one chamber, the second spring configured to apply a second force to a deformable sealing member towards the column end fitting; and a pushing and latching mechanism configured to push the at least one body member and the first and second springs towards the column end fitting.

Abstract translation: 用于将包括端部配件的液相色谱柱耦合到管道的装置包括：至少一个主体构件，其包括至少一个室; 在所述至少一个室内的第一弹簧，所述第一弹簧被构造成对所述管道朝向所述柱端配件施加第一力; 在所述至少一个室内的第二弹簧，所述第二弹簧构造成朝向所述柱端配件向可变形密封构件施加第二力; 以及推动和闭锁机构，其构造成将所述至少一个本体构件和所述第一和第二弹簧推向所述柱端配件。

公开(公告)号：WO2013062624A1

公开(公告)日：2013-05-02

申请号：PCT/US2012/034895

申请日：2012-04-25

Applicant: THERMO FINNIGAN LLC ,  SONG, Tao ,  BRANN, John, E. ,  TAKAREWSKI, Joseph, John ,  FRANCE, Eric, A.

Inventor： SONG, Tao ,  BRANN, John, E. ,  TAKAREWSKI, Joseph, John ,  FRANCE, Eric, A.

IPC: G01N30/02

CPC classification number: G01M3/28 ,  G01N30/32 ,  G01N30/88 ,  G01N2030/889

Abstract: A method for monitoring a fluidic system of a liquid chromatography system is characterized by: (a) drawing a fluid into a syringe pump; (b) configuring a valve so as to fluidically couple the pump to either a fluidic pathway through the fluidic system or to a plug that prevents fluid flow; (c) causing the syringe pump to progressively compress the fluid therein or expel the fluid to the fluidic pathway, while measuring a pressure of the fluid; (d) determining a profile of the variation of the measured pressure; (e) comparing the determined profile to an expected profile that depends upon the fluid; and (f) providing a notification of a sub-optimal operating condition or malfunction if the determined profile varies from the expected profile by greater than a predetermined tolerance.

Abstract translation: 用于监测液相色谱系统的流体系统的方法的特征在于：（a）将流体抽入注射器泵; （b）构造阀，以将流体地耦合到通过流体系统的流体路径或阻止流体流动的塞子; （c）在测量流体的压力的同时使注射器泵逐渐压缩其中的流体或将流体排出到流体通路; （d）确定测量压力的变化的轮廓; （e）将确定的轮廓与取决于流体的预期轮廓进行比较; 以及（f）如果所确定的轮廓与所述预期轮廓相比大于预定的公差，则提供非最佳操作条件或故障的通知。

公开(公告)号：WO2012058516A1

公开(公告)日：2012-05-03

申请号：PCT/US2011/058230

申请日：2011-10-28

Applicant: THERMO FINNIGAN LLC ,  BRANN, John, E.

Inventor： BRANN, John, E.

IPC: B01D15/08 ,  G01N30/00

CPC classification number: B01D15/14 ,  B01D15/16 ,  B01D15/163 ,  B01D15/424 ,  B01D35/1435 ,  G01M3/28 ,  G01M3/3272 ,  G01N30/20 ,  G01N30/24 ,  G01N30/32 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2030/347 ,  G01N2035/009 ,  Y10T137/85978

Abstract: A system for providing a solvent or reagent to a liquid chromatography system comprises: a valve comprising a common port and a plurality of other ports, configurable such that the common port may be fluidically coupled to any one of the other ports; a pump fluidically coupled to the common port of the valve; a plug configured to block flow through a first one of said other ports of the valve; a container containing the solvent or reagent, said container fluidically coupled to a second one of said other ports of the valve; a fluid tubing line having a known resistance to fluid flow fluidically coupled to a third one of said other ports of the valve; and a pressure gauge configured to measure fluid pressure within the pump, wherein the solvent or reagent is provided to the liquid chromatography system by a fourth one of the other ports.

Abstract translation: 用于向液相色谱系统提供溶剂或试剂的系统包括：阀，其包括公共端口和多个其它端口，可配置为使得公共端口可流体耦合到任何其它端口; 泵，其流体地联接到所述阀的公共端口; 栓塞，被配置为阻止通过所述阀的所述另一个端口中的第一个的流动; 容纳所述溶剂或试剂的容器，所述容器流体耦合到所述阀的所述其它端口中的第二端口; 具有流体连接到所述阀的所述另一个端口中的第三个的流体流动的已知阻力的流体管线; 以及配置成测量所述泵内的流体压力的压力计，其中所述溶剂或试剂由所述其它端口中的第四个提供给所述液相色谱系统。

公开(公告)号：WO2012058515A3

公开(公告)日：2012-05-03

申请号：PCT/US2011/058229

申请日：2011-10-28

Applicant: THERMO FINNIGAN LLC ,  BRANN, John, E.

Inventor： BRANN, John, E.

IPC: B01D15/08 ,  G01N30/00

Abstract: A cartridge for chromatography separations comprises a housing; at least a first and a second chromatography column at least partially passing through the housing, each chromatography column comprising a first and a second end fitting operable to connect the chromatography to external tubing of a chromatography system. Connection fittings protruding outside of the housing at each end of each column enable fluidic connection to a chromatograph plumbing system and/or mass spectrometer detector. The cartridge may comprise heaters and temperature sensors and optional sensors to monitor fluid flow rate or pH. A passive identification feature, e.g., a barcode or RFID module may identify the cartridge and its associated chromatography methods to external software. Further, an on-board memory module and controller chip may be used to actively record computer-readable module information, including module history information, the information transferable through a standard interface, such as a universal serial bus (USB) port.

公开(公告)号：WO2016099979A1

公开(公告)日：2016-06-23

申请号：PCT/US2015/064268

申请日：2015-12-07

Applicant: THERMO FINNIGAN LLC

Inventor： BRANN, John, E. ,  TAKAREWSKI, Joseph, John

IPC: G01N33/80 ,  G01N1/44

CPC classification number: G01N33/492 ,  G01N1/44 ,  G01N30/7233 ,  G01N30/88 ,  G01N33/491 ,  G01N33/80 ,  G01N35/1016 ,  G01N35/1097 ,  G01N2030/8822 ,  G01N2035/00475

Abstract: A method for analyzing a whole blood sample can include injecting whole blood into a first zone of a dual zone sample loop, applying sufficient heat or energy to the whole blood to disintegrate the cellular components of the whole blood sample to produce cell disintegrated blood, and injecting a sufficient volume of the buffer into the dual zone sample loop to move the cell disintegrated blood into a second zone of the dual zone sample loop. The method can further include switching a multiport value to an inject position, flowing the cell disintegrated blood from the dual zone sample loop into a solid phase extraction column, and eluting components of the cell disintegrated blood from the solid phase extraction column into a liquid chromatography column.

Abstract translation: 用于分析全血样品的方法可以包括将全血注射到双区样品环的第一区域中，向全血施加足够的热量或能量以使整个血液样品的细胞组分崩解以产生细胞分解的血液，以及 将足够体积的缓冲液注射到双区样品环中以将细胞分解的血液移动到双区样品环的第二区。 该方法还可以包括将多端口值切换到注入位置，使细胞分解的血液从双区样品环流入固相萃取柱，以及将细胞分解的血液从固相萃取柱中洗脱成分成液相色谱 柱。