公开(公告)号：WO2021019275A1

公开(公告)日：2021-02-04

申请号：PCT/IB2019/056397

申请日：2019-07-26

Applicant: UNIVERSITA' DEGLI STUDI DI CAGLIARI

Inventor： PIANO, Dario ,  FARCI, Domenica

IPC: B01D15/34 ,  A23L5/44 ,  A23L33/105 ,  C12P23/00 ,  C07C403/24 ,  B01D11/02 ,  C12M1/26 ,  B01D61/14

Abstract: The present invention relates to a method for the production of chromoplastid carotenoids that can be isolated from fruits. The procedure of the invention allows to selectively isolate, on the basis of the size (Size Exclusion Chromatography) and in pure form, the carotenoid in the form of regularly shaped and sized aggregates. The process includes the following steps: i) production, in suspension, of homogeneous fragments of the chromoplastidial membranes from the fruits; ii) solution isolation of the membrane components by solubilization with detergents; iii) selective and high-purity isolation of the carotenoid in the form of regular paracrystalline aggregates by a screening technique such as size exclusion chromatography (SEC - Size Exclusion Chromatography).