公开(公告)号：WO2022032002A1

公开(公告)日：2022-02-10

申请号：PCT/US2021/044799

申请日：2021-08-05

Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED

Inventor： GUO, Zhongwu ,  BAILEY, Laura S. ,  BASSO, Kari B. ,  HUANG, Fanran ,  GAO, Tianqi ,  ZHAO, Jinying

IPC: G06F19/00 ,  H01J49/26 ,  G16B10/00

Abstract: Mass spectrometry (MS) based systems and methods of implementing multi-stage MS/MS analysis for identification of experimental glycolipid samples are described. A processor communicatively coupled to a memory and a reference spectral database (1) implements a first stage analysis of glycan fragment data including determining, based on a matching reference glycan of a candidate reference glycolipid, a glycan structure of a glycan portion of the experimental glycolipid. The processor further (2) implements a second stage analysis of lipid fragment data including performing a spectral comparison of one or more MS/MS spectral values of a shifted experimental lipid fragment data with one or more MS/MS spectral values of matching reference lipid fragment data of the candidate reference glycolipid. A combination of the determined glycan fragments and lipid fragments gives the structure of each specific glycolipid in the experimental glycolipid sample.