公开(公告)号：WO2018144906A1

公开(公告)日：2018-08-09

申请号：PCT/US2018/016693

申请日：2018-02-02

Applicant: UNIVERSITY OF MARYLAND, COLLEGE PARK

Inventor： DEVOE, Don L ,  SPOSITO, Alex

IPC: B01L3/00 ,  C12Q1/68

Abstract: A microfluidic device including at least one channel in fluid communication with a sample trap array. Specifically, the configuration and geometry of the trap arrays according to the present invention allows for performing sample digitization that supports passive self-discretization within the sample traps without the need for any external flow control or actuation. Geometrical parameters defining the sample traps, including the trap width and the trap depth, are selected to optimize self-filling of the sample traps. Reagents are incorporated into the sample traps during device fabrication to allow for performing multiplexed reactions within the sample traps.

公开(公告)号：WO2017181069A1

公开(公告)日：2017-10-19

申请号：PCT/US2017/027706

申请日：2017-04-14

Applicant: UNIVERSITY OF MARYLAND, COLLEGE PARK

Inventor： DEVOE, Donald ,  KENDALL, Eric ,  SPOSITO, Alex

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  G01F1/68 ,  G01N30/88 ,  H05B3/14

CPC classification number: B01L7/52 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0688 ,  C12Q1/686 ,  C12Q2527/101 ,  C12Q2565/629

Abstract: The present invention relates to a microfluidic system including a temperature controller and a thermoplastic microfluidic chip that enables rapid PCR in a PCR chamber of the microfluidic chip. Thermal control of the PCR chamber is achieved by applying voltage to heater electrodes patterned directly onto one layer of the microfluidic chip. The temperature controller adjusts the voltage applied to the heater electrodes by changing temperature controller parameters selected to minimize duration of each PCR cycle. Furthermore, simple operation of the microfluidic chip is provided through using an integrated passive capillary valve, requiring minimum operator intervention and eliminating the need for fluidic interfacing, pumping, or metering during chip loading.

Abstract translation: 本发明涉及包括温度控制器和热塑性微流体芯片的微流体系统，其能够在微流体芯片的PCR室中进行快速PCR。 通过将电压施加到直接图案化到微流体芯片的一层上的加热器电极来实现PCR腔室的热控制。 温度控制器通过改变选定的温度控制器参数来调整施加到加热器电极的电压，以最小化每个PCR循环的持续时间。 此外，微流控芯片的简单操作通过使用集成无源毛细管阀门提供，只需最少的操作员干预，并且在芯片加载过程中不需要流体接口，泵送或计量。