公开(公告)号：WO2020061296A1

公开(公告)日：2020-03-26

申请号：PCT/US2019/051907

申请日：2019-09-19

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： GETHINGS, Lee ,  ISAAC, Giorgis, M. ,  MUNJOMA, Nyasha, Clarence ,  PLUMB, Robert, S. ,  RAINVILLE, Paul

IPC: B01D15/30 ,  G01N30/88 ,  G01N30/72 ,  G01N33/92

Abstract: The present disclosure relates to methodologies, systems, and devices for screening lipids. The technique includes selecting a set of standards to identify at least one desired class of lipids, spiking the standards into a biological sample to form a sample matrix, extracting the lipids from the sample matrix, and introducing the sample matrix into a chromatography system to separate the desired class of lipids. Once the lipids are separated into different lipid classes using HILIC chromatography, they are directed to a detector, and the separated lipids are quantified based on a comparison between the measured detector response and a calibration curve generated with a known concentration of the selected set of standards.

公开(公告)号：WO2018067398A1

公开(公告)日：2018-04-12

申请号：PCT/US2017/054389

申请日：2017-09-29

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： RAINVILLE, Paul ,  LAUBER, Matthew, A. ,  GETHINGS, Lee ,  PLUMB, Robert, S. ,  COSGRAVE, Eoin ,  BROUSMICHE, Darryl, W.

IPC: G01N33/68

Abstract: Provided herein are methodologies where a glycosylated protein or peptide is subjected to peptide bond cleavage to produce a glycan amino acid complex wherein the N-linked or O- linked glycan is attached. A derivatization reagent is then attached to the N terminus of the amino acid to provide a labeled glycan amino acid complex. The labeled glycan amino acid complex is then separated from the matrix via one or more methods including HILIC SPE, and injected directly onto an LC or LC/MS system for analysis, detection and characterization of the glycosylated protein or the peptide.

Abstract translation: 本文提供了糖基化蛋白质或肽进行肽键裂解以产生其中N-连接的或O-连接的聚糖连接的聚糖氨基酸复合物的方法。 然后将衍生化试剂连接到氨基酸的N末端以提供标记的聚糖氨基酸复合物。 然后通过包括HILIC SPE的一种或多种方法将标记的聚糖氨基酸复合物与基质分离，并将其直接注射到LC或LC / MS系统上用于糖基化蛋白质或肽的分析，检测和表征。