公开(公告)号：WO2019236620A1

公开(公告)日：2019-12-12

申请号：PCT/US2019/035447

申请日：2019-06-04

申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人： GE, Ying ,  JIN, Song ,  GUARDADO ALVAREZ, Tania ,  BROWN, Kyle

IPC分类号： B05D3/00 ,  B05D5/06 ,  B32B9/00

摘要： The present invention provides photo-cleavable anionic surfactants, particularly 4-hexylphenylazosulfonate (Azo) and sodium 4-hexylphenylazosulfonate derivatives, which can be rapidly degraded upon UV irradiation, for top-down and bottom-up proteomics. These surfactants can effectively solubilize proteins and peptide fragments with performance comparable to sodium dodecyl sulfate (SDS) and are compatible with mass spectrometry analysis of the solubilized proteins and peptide fragments. Top-down proteomic studies using the present photo-cleavable anionic surfactants has allowed the detection of 100-fold more unique proteoforms as compared to controls and has enabled the solubilization of membrane proteins for comprehensive characterization of protein post-translational modifications. In addition, the present photo-cleavable anionic surfactants are also suitable for dissolving polypeptides in bottom-up proteomic experiments including extracellular matrix proteomics, and are suitable as a substitute for SDS in gel electrophoresis.

公开(公告)号：WO2021127434A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/066011

申请日：2020-12-18

申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人： GE, Ying ,  JIN, Song ,  TIAMBENG, Timothy-Neil ,  ROBERTS, David

IPC分类号： A61K9/14 ,  A61K9/50 ,  A61K33/26 ,  C12N15/87 ,  H01F1/375

摘要： This invention provides mass spectrometry (MS) compatible nanomaterials for the selective capture and enrichment of low abundance proteins as well as MS analysis of different proteoforms of proteins, particularly cardiac proteins and different proteoforms of cardiac troponin I (cTnI) arising from post-translational modifications and sequence variations. The surface of superparamagnetic nanoparticles is functionalized with probe molecules that specifically bind to the desired protein. In an embodiment, the nanoparticles are functionalized with probe molecules having high affinity and selectivity for cTnI within the human cardiac troponin complex. This allows for MS-analysis and characterization of cTnI proteoforms from human heart tissue lysates and human blood or serum samples, and provides an accurate assay for detection of cTnI with molecular details. Such assays are useful for accurate diagnosis of acute coronary syndrome and chronic diseases, including acute myocardial infarction and other cardiac injuries, as well as risk stratification and outcome assessment for patients.

公开(公告)号：WO2010036520A1

公开(公告)日：2010-04-01

申请号：PCT/US2009/056632

申请日：2009-09-11

申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION ,  JIN, Song ,  GE, Ying ,  NELSON, Cory, Alexander ,  XU, Qingge

发明人： JIN, Song ,  GE, Ying ,  NELSON, Cory, Alexander ,  XU, Qingge

IPC分类号： C07K1/22

CPC分类号： C07K1/22

摘要： The present invention provides methods and materials for isolating, purifying, and/or enriching the concentration of compounds having one or more phosphate groups and/or derivatives thereof, including but not limited to phosphorylated peptides and/or phosphorylated proteins. In some aspects, the present invention provides nanostructured enrichment materials, such as metal oxide mesoporous materials, that selectively and reversibly bind with phosphorylated compounds with high specificity and are capable of controlled release of phosphorylated compounds bound to their active surfaces. Mesoporous materials of the present invention also provide enrichment materials having large active surface areas that provide for higher loading capacities for phosphorylated peptides and proteins relative to conventional affinity based methods. Nanostructured metal oxide mesoporous enrichment materials of the present invention are also compatible with implementation via a variety of separation platforms including flow through separation systems, elution based separation systems, column chromatography and affinity chromatography.

摘要翻译： 本发明提供用于分离，纯化和/或富集具有一个或多个磷酸基团和/或其衍生物的化合物的浓度的方法和材料，包括但不限于磷酸化肽和/或磷酸化蛋白质。 在一些方面，本发明提供纳米结构富集材料，例如金属氧化物介孔材料，其与高度特异性的磷酸化化合物选择性和可逆结合，并且能够控制释放与其活性表面结合的磷酸化化合物。 本发明的介孔材料还提供具有大的活性表面积的富集材料，相对于常规的基于亲和力的方法，磷酸化肽和蛋白质具有更高的负载能力。 本发明的纳米结构金属氧化物介孔富集材料还可通过各种分离平台实施，包括流通分离系统，基于洗脱的分离系统，柱色谱和亲和层析。