公开(公告)号：WO2023075623A1

公开(公告)日：2023-05-04

申请号：PCT/PT2022/050028

申请日：2022-10-26

Applicant: INEB (INSTITUTO NACIONAL DE ENGENHARIA BIOMÉDICA) ,  UNIVERSIDADE DO PORTO

Inventor： DOS SANTOS CUNHA, Carla Marisa ,  DOS SANTOS BARBER, Susana Gomes ,  MONTEIRO DA ROCHA BARBOSA, Mário Adolfo ,  DA SILVA PEREIRA, Paulo Miguel

IPC: C12N5/0786 ,  A61P19/02 ,  A61P29/00 ,  A61K35/15 ,  A61K48/00 ,  C12N2501/052 ,  C12N2501/15 ,  C12N2501/22 ,  C12N2501/2304 ,  C12N2501/2306 ,  C12N2501/231 ,  C12N2501/2313 ,  C12N2501/24 ,  C12N2501/25 ,  C12N2506/115 ,  C12N5/0645

Abstract: The present invention refers to monocytes and macrophages for use in the treatment of intravertebral disc (IVD) herniation. Furthermore, the macrophage cell phenotype which is most effective for use in a phagocytosis immunotherapy is herein disclosed. Thus, the present invention provides compositions comprising such macrophage phenotypes and a method for differentiation, polarization, and purification of the most effective macrophage phenotype, as well as its preferred form of administration. Another aspect of the present invention refers to the compositions employed in the said polarization of the most effective macrophage phenotype. Yet another aspect of the present invention refers to the compositions employed in the purification of the most effective macrophage phenotype through negative selection. The uses, compositions and methods of the present invention can be advantageously employed in a cell-based therapy primarily for use in the treatment of intervertebral disc (IVD) herniation, as well as for other clinical conditions improved by the regression of regeneration-adverse reactive tissues, such as wound and surgery- related scar tissue, fibrosis and necrosis; the said clinical condition being selected from the list further consisting of myocardial infraction, endometriosis, pulmonary asthma, hepatic cirrhosis, spinal cord injury and cartilage injury.