活体营养型病原物
1. 一种获得活体营养型植物病原卵菌无污染基因组的方法
申请号: CN201911223140.4
申请日: 2019-12-03
公开（公告）号: CN111028889B
公开（公告）日: 2021-04-20
发明人: 尹玲; 曲俊杰
本发明公开了一种获得活体营养型植物病原卵菌无污染基因组的方法：先鉴定污染源，从NCBI下载候选污染物种对应的全基因组序列，与高通量测序得到的待组装的原始reads比对，去除对比到污染源的reads，得到初步组装结果，再将其分为多个窗口，统计每个窗口的基因组区域的深度平均值和GC含量，通过GC分布将植物病原卵菌和污染源物种基因组的窗口区域区分开，进而获得污染源物种基因组对应的窗口区域，去除map到污染源物种基因组窗口区域的reads序列，保留剩余的reads，组装后得到最终的组装结果。从根本上保证获得的活体营养型植物病原卵菌基因组序列信息的完整性和准确性，为后续致病相关基因、病原卵菌致病机制等的进一步研究，提供了有力保证。
更多2. 一种获得活体营养型植物病原卵菌无污染基因组的方法
申请号: CN201911223140.4
申请日: 2019-12-03
公开（公告）号: CN111028889A
公开（公告）日: 2020-04-17
发明人: 尹玲; 曲俊杰
本发明公开了一种获得活体营养型植物病原卵菌无污染基因组的方法：先鉴定污染源，从NCBI下载候选污染物种对应的全基因组序列，与高通量测序得到的待组装的原始reads比对，去除对比到污染源的reads，得到初步组装结果，再将其分为多个窗口，统计每个窗口的基因组区域的深度平均值和GC含量，通过GC分布将植物病原卵菌和污染源物种基因组的窗口区域区分开，进而获得污染源物种基因组对应的窗口区域，去除map到污染源物种基因组窗口区域的reads序列，保留剩余的reads，组装后得到最终的组装结果。从根本上保证获得的活体营养型植物病原卵菌基因组序列信息的完整性和准确性，为后续致病相关基因、病原卵菌致病机制等的进一步研究，提供了有力保证。
更多3. 用于预防或降低细菌和真菌病原体对作物植物的感染的方法和组合物
申请号: CN201580073784.3
申请日: 2015-11-25
公开（公告）号: CN107208112A
公开（公告）日: 2017-09-26
发明人: D·W·加布里埃尔; S·张
本发明教导了可用于治疗、预防或治愈活体植物的病原体感染的方法和组合物。具体而言，本发明教导了增强植物对病原体相关分子模式的响应的方法。本文所述的方法和组合物对治疗活体营养型病原体(包括韧皮部杆菌(Liberibacter))是有效的。具体而言，该组合物和方法可用于保护植物免受细胞内和细胞间的细菌和真菌攻击和感染，包括Bcl‑2相关抗凋亡基因6(BAG6)基因的沉默。
更多4. 锈病抗性基因
申请号: CN201480052048.5
申请日: 2014-08-21
公开（公告）号: CN105612255A
公开（公告）日: 2016-05-25
发明人: E·拉吉达; J·W·摩尔
本发明涉及用于赋予植物对一个或多个活体营养性病原真菌抗性的新型转运多肽及其编码基因。
更多5. 具有对植物病原体增强的抗性的植物以及用于在植物中产生增强的病原体抗性的方法
申请号: CN201580061046.7
申请日: 2015-11-05
公开（公告）号: CN107109425A
公开（公告）日: 2017-08-29
发明人: 加百利·斯查弗; 德巴布拉特·拉哈; 马克·弗里尔; 马里利亚·K·F·德坎波斯; 菲利普·约恩; 萨斯基亚·C·M·凡维斯
本发明涉及对植物病原体具有增强的抗性的植物，其中与野生型植物相比，在所述植物中肌醇焦磷酸盐InsP7和/或InsP8的细胞内浓度是增加的。具体地，本发明涉及具有至少一种参与合成肌醇焦磷酸盐InsP7和/或InsP8的蛋白质(例如蛋白质VIH2和VIH1)的增加的表达的植物。根据本发明的植物对以下植物病原体特别地有抗性：食草性昆虫，例如农业相关害虫的幼虫；致病真菌，如死体营养性真菌；或其他植物害虫，如活体营养性病原体。本发明还涉及用于增强对植物病原体的植物抗性的方法，其中与野生型植物相比，肌醇焦磷酸盐lnsP7和/或InsP8的细胞内浓度是增加的。
更多6. AtHIR4基因和/或其编码蛋白在调控植物病原菌抗性中的应用
申请号: CN202410674410.8
申请日: 2024-05-28
公开（公告）号: CN118496330A
公开（公告）日: 2024-08-16
发明人: 程家森; 刘小凡; 陈桃; 谢甲涛; 付艳苹; 姜道宏; 李博
本发明属于基因工程技术领域，具体涉及AtHIR4基因和/或其编码蛋白在调控植物病原菌抗性中的应用。本发明所述AtHIR4基因编码的蛋白的氨基酸序列如SEQ ID No.1所示。本发明创建了超量表达超敏反应诱导蛋白4(AtHIR4)的转基因拟南芥和油菜，其中AtHIR4转基因拟南芥中，由几丁质激活的ROS爆发和MAPKs的磷酸化均明显增强，抗病性检测结果表明AtHIR4转基因植物不仅对死体营养型病原真菌核盘菌和灰霉的抗性增强，而且对半活体营养型病原细菌丁香假单胞菌的抗病性也显著增加；AtHIR4转基因油菜对核盘菌和灰霉病的抗性也显著增强。
更多7. 一种抗根肿菌的几丁质酶及编码基因和应用
申请号: CN202210984055.5
申请日: 2022-08-17
公开（公告）号: CN116004584B
公开（公告）日: 2024-04-02
发明人: 陈桃; 赵艳丽; 谢甲涛; 姜道宏; 付艳苹; 程家森
本发明属于植物基因工程技术领域，具体涉及一种抗根肿菌的几丁质酶及编码基因和应用。本所述几丁质酶PbChia1来源于根肿菌，体外表达的PbChia1蛋白可以抑制根肿菌休眠孢子的萌发，延缓根肿菌的发育，缓解油菜根肿病根肿的症状，生防效果为61.29％。超表达PbChia1的转基因植物可以显著降低根肿菌在植物内的定殖率，延缓根肿菌的发育，对根肿病的生防效果高达62.3％。转基因植株对活体营养型病原丁香假单胞菌PstDC3000及死体营养型病原核盘菌和立枯丝核菌均显示抗病能力。表明根肿菌几丁质酶PbChia1可为根肿病的绿色防控提供新基因资源。
更多8. 一种抗根肿菌的几丁质酶及编码基因和应用
申请号: CN202210984055.5
申请日: 2022-08-17
公开（公告）号: CN116004584A
公开（公告）日: 2023-04-25
发明人: 陈桃; 赵艳丽; 谢甲涛; 姜道宏; 付艳苹; 程家森
本发明属于植物基因工程技术领域，具体涉及一种抗根肿菌的几丁质酶及编码基因和应用。本所述几丁质酶PbChia1来源于根肿菌，体外表达的PbChia1蛋白可以抑制根肿菌休眠孢子的萌发，延缓根肿菌的发育，缓解油菜根肿病根肿的症状，生防效果为61.29％。超表达PbChia1的转基因植物可以显著降低根肿菌在植物内的定殖率，延缓根肿菌的发育，对根肿病的生防效果高达62.3％。转基因植株对活体营养型病原丁香假单胞菌PstDC3000及死体营养型病原核盘菌和立枯丝核菌均显示抗病能力。表明根肿菌几丁质酶PbChia1可为根肿病的绿色防控提供新基因资源。
更多9. 活体果蔬专用生态基质及商品化生产方法
申请号: CN201110150201.6
申请日: 2011-06-07
公开（公告）号: CN102285828A
公开（公告）日: 2011-12-21
发明人: 卢红
本发明涉及农业科技领域，一种新型的活体果蔬专用生态基质及商品化生产方法。其特征在于：本发明公开了活体果蔬专用生态基质的概念与商品化实现方法，具有配方独特专有且工艺流程简约的特点。以生物生态链技术为出发点，用10％蚯蚓和蛆虫等剁绞的肉泥混合近90％的蚯蚓粪便蛆虫粪便，添加复合生物菌剂二次发酵，有效杀灭病原微生物和寄生虫后，直接用于活体果蔬的有机栽培。不含化肥农药，也不含有害于环境和身体健康的物质，但能提供活体果蔬生命周期中必需的均衡营养元素。活体果蔬播种于装有专用生态基质的可再生托盘中，置于多层立体架上自动控制喷施补充水分，即可实现标准化规模化工厂化生产优质优价高产的绿色安全的有机活体果蔬。实行会员制销售模式。农业增效，农民增收。
更多10. ISOLATION AND CHARACTERISATION OF THE CSB3-1 MUTANT OF ARABIDOPSIS THALIANA AND USE THEREOF AS A REGULATOR OF RESISTANCE IN PLANTS TO DISEASES CAUSED BY BIOTROPHIC PATHOGENS
申请号: EP06764380.9
申请日: 2006-06-15
公开（公告）号: EP1908838A2
公开（公告）日: 2008-04-09
发明人: Gil Morrio, María J., Inst. de Biología Molecular; Coego Gonzalez, Berto, Inst. de Biología Molecular; Vera Vera, Pablo, Inst. de Biología Molecular
The invention relates to the technical field of plant biotechnology and, more specifically, to the identification and characterization of the csb3-1 mutant of Arabidopsis thaliana, as well as to the use thereof in the regulation of resistance to diseases caused by biotrophic pathogens and to the applications of same in the generation of plants that are resistant to said type of pathogens.
11. Amino acid transport protein and gene thereof
申请号: JP2010264656
申请日: 2010-11-29
公开（公告）号: JP2011084564A
公开（公告）日: 2011-04-28
发明人: ENDO HITOSHI; KANAI YOSHIKATSU
PROBLEM TO BE SOLVED: To provide a novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. SOLUTION: Intensive studies were made to identify a tumor cell membrane surface molecule conjugating or interacting with a cell membrane surface 4F2 molecule seemingly playing an important role in the activation of an unknown amino acid transporter. As a result, a gene encoding the novel amino acid transporter molecule, which mediates the incorporation of various neutral amino acids, various drugs or physiological substances into cells, has been found out and a substance capable of inhibiting the incorporation of amino acids via this molecule and thus inhibiting the proliferation of tumor cells has been also found out. COPYRIGHT: (C)2011,JPO&INPIT
更多12. Neutral amino acid transporter and gene thereof
申请号: US09786389
申请日: 1999-09-03
公开（公告）号: US07332572B1
公开（公告）日: 2008-02-19
发明人: Hitoshi Endou; Yoshikatsu Kanai
A novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the above amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. Intensive studies were made to identify a tumor cell membrane surface molecule associating or interacting with a cell membrane surface 4F2hc molecule seemingly playing an important role in the activation of an unknown amino acid transporter. As a result, a gene encoding the novel amino acid transporter molecule, which mediates the incorporation of various neutral amino acids, various drugs or physiological substances into cells, has been found out and a substance capable of inhibiting the incorporation of amino acids via this molecule and thus inhibiting the proliferation of tumor cells has been also found out.
更多13. Neutral amino acid transporter and gene thereof
申请号: US10912983
申请日: 2004-08-05
公开（公告）号: US20050064475A1
公开（公告）日: 2005-03-24
发明人: Hitoshi Endou; Yoshikatsu Kanai
A novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the above amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. Intensive studies were made to identify a tumor cell membrane surface molecule associating or interacting with a cell membrane surface 4F2hc molecule seemingly playing an important role in the activation of an unknown amino acid transporter. As a result, a gene encoding the novel amino acid transporter molecule, which mediates the incorporation of various neutral amino acids, various drugs or physiological substances into cells, has been found out and a substance capable of inhibiting the incorporation of amino acids via this molecule and thus inhibiting the proliferation of tumor cells has been also found out.
更多14. Neutral amino acid transporter and gene thereof
申请号: US10913191
申请日: 2004-08-05
公开（公告）号: US07666999B2
公开（公告）日: 2010-02-23
发明人: Hitoshi Endou; Yoshikatsu Kanai
A novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the above amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. Intensive studies were made to identify a tumor cell membrane surface molecule associating or interacting with a cell membrane surface 4F2hc molecule seemingly playing an important role in the activation of an unknown amino acid transporter. As a result, a gene encoding the novel amino acid transporter molecule, which mediates the incorporation of various neutral amino acids, various drugs or physiological substances into cells, has been found out and a substance capable of inhibiting the incorporation of amino acids via this molecule and thus inhibiting the proliferation of tumor cells has been also found out.
更多15. Method using neutral amino acid transporter expressing cell
申请号: US10913263
申请日: 2004-08-05
公开（公告）号: US07335482B2
公开（公告）日: 2008-02-26
发明人: Hitoshi Endou; Yoshikatsu Kanai
A novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the above amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. Intensive studies were made to identify a tumor cell membrane surface molecule associating or interacting with a cell membrane surface 4F2hc molecule seemingly playing an important role in the activation of an unknown amino acid transporter. As a result, a gene encoding the novel amino acid transporter molecule, which mediates the incorporation of various neutral amino acids, various drugs or physiological substances into cells, has been found out and a substance capable of inhibiting the incorporation of amino acids via this molecule and thus inhibiting the proliferation of tumor cells has been also found out.
更多16. DNA encoding neutral amino acid transporter
申请号: US10913256
申请日: 2004-08-05
公开（公告）号: US07413895B2
公开（公告）日: 2008-08-19
发明人: Hitoshi Endou; Yoshikatsu Kanai
A novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the above amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. Intensive studies were made to identify a tumor cell membrane surface molecule associating or interacting with a cell membrane surface 4F2hc molecule seemingly playing an important role in the activation of an unknown amino acid transporter. As a result, a gene encoding the novel amino acid transporter molecule, which mediates the incorporation of various neutral amino acids, various drugs or physiological substances into cells, has been found out and a substance capable of inhibiting the incorporation of amino acids via this molecule and thus inhibiting the proliferation of tumor cells has been also found out.
更多17. Neutral amino acid transporter binding method
申请号: US10912983
申请日: 2004-08-05
公开（公告）号: US07332293B2
公开（公告）日: 2008-02-19
发明人: Hitoshi Endou; Yoshikatsu Kanai
A novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the above amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. Intensive studies were made to identify a tumor cell membrane surface molecule associating or interacting with a cell membrane surface 4F2hc molecule seemingly playing an important role in the activation of an unknown amino acid transporter. As a result, a gene encoding the novel amino acid transporter molecule, which mediates the incorporation of various neutral amino acids, various drugs or physiological substances into cells, has been found out and a substance capable of inhibiting the incorporation of amino acids via this molecule and thus inhibiting the proliferation of tumor cells has been also found out.
更多18. NEUTRAL AMINO ACID TRANSPORTER AND GENE THEREOF
申请号: EP99940648.1
申请日: 1999-09-03
公开（公告）号: EP1111048A2
公开（公告）日: 2001-06-27
发明人: ENDOU, Hitoshi; KANAI, Yoshikatsu
A novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the above amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. Intensive studies were made to identify a tumor cell membrane surface molecule associating or interacting with a cell membrane surface 4F2hc molecule seemingly playing an important role in the activation of an unknown amino acid transporter. As a result, a gene encoding the novel amino acid transporter molecule, which mediates the incorporation of various neutral amino acids, various drugs or physiological substances into cells, has been found out and a substance capable of inhibiting the incorporation of amino acids via this molecule and thus inhibiting the proliferation of tumor cells has been also found out.
