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发明公开
CN101031652A 4－氨基－4－去氧分支酸盐/酯(ADC)和[3R,4R]－4－氨基－3－羟基环己－1,5－二烯－1－羧酸（3,4－CHA）的生物合成生产 无效 - 撤回 转让

4－氨基－4－去氧分支酸盐/酯(ADC)和[3R,4R]－4－氨基－3－羟基环己－1,5－二烯－1－羧酸（3,4－CHA）的生物合成生产
摘要：
本发明涉及一种方法，用于对4－氨基－4－去氧分支酸盐/酯(ADC)进行生物合成生产，所述方法通过用4－氨基－4－去氧分支酸合酶，优选地，PabAB二分蛋白(其可以是融合蛋白)以增加的活性水平开展体内发酵来进行，由此获得包含ADC和4－氨基－4－去氧预苯酸盐/酯(ADP)的培养液，对它们进行回收。本发明还涉及另一种方法，将ADP转化为对氨基苯丙氨酸。此外，本发明还涉及对[3R，4R]－4－氨基－3－羟基环己－1，5－二烯－1－羧酸(3，4－CHA)的生物合成生产，这是通过上述4－氨基－4－去氧分支酸合酶和能将异分支酸盐/酯转化为[5S，6S]－5，6－二羟基环己－1，3－二烯－1－羧酸(2，3－CHD)的酶(优选吩嗪生物合成蛋白PhzD)的协调作用来进行的，还包括回收3，4－CHA。本发明还涉及用于此类方法的载体和宿主细胞。本发明还涉及3，4－CHA用作为催化活性产品，尤其是作为手性催化剂的用途。本发明最后还涉及从3，4－CHA合成磷酸奥司他韦的方法。
摘要（英）：

The invention relates to a process for the biosynthetic production of 4-amino-4-deoxychorismate (ADC) performed fermentatively in vivo with a 4-amino-4-deoxychorismate synthase, preferably a PabAB bipartite protein (which may be a fusion protein), at an increased level of activity, thereby obtaining a broth comprising ADC and 4-amino-4-deoxyprephenate (ADP), that are recovered. The invention also relates to a further process of converting the ADP into p-aminophenylalanine. The invention, moreover relates to biosynthetic production of [3 R ,4 R ]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA), by concerted action of such 4-amino-4-deoxychorismate synthase and of an enzyme capable of converting isochorismate into [5 S ,6 S ]-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylic acid (2,3-CHD), preferably a phenazine biosynthesis protein PhzD, including recovery of 3,4-CHA. The invention also relates to expression vectors and host cells for use in any of such processes. And the invention finally relates to synthesis of oseltamivir phosphate from 3,4-CHA.

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