The invention discloses a rolling load for a method of culturing microorganism and a bioreactor, which comprises the following parts: a culturing cell incubator with controlling O2, CO2 and temperature, a bottom board of culturing room fixing the culture in the condition of keeping fresh perfusion of culturing solution, a roll pressure load applying the surface of the culture, wherein, the roll speed is 0. 1cm/s-15cm/s according to physiological load; the reciprocal roll frequency of loading body is 0. 1HZ-3HZ; the loading time is 1-3 times per 24h and every time is 10-180min, the culturing time is 1-60 days, the culture changes 5%-35% at the roll load. The invention accesses the pressure environment of some organization growth, which is fit for the growth of the cell and the organization, improves the content of the cartilage cell.