The present invention belongs to the field of animal propagation technology, in the concrete, said invention belongs to the field of poultry sex identification technology, in particular it relates to a method for identifying chick embryonic desc sex by utilizing multiple PCR. Said method includes the following steps: (1), according to chick EE0.6 sequence whose GenBank accession number is D85614 designing two pairs of nested primers and according to chick wispl gene whose GenBak accession number is NC 006089 designing two pairs of nested primers which can be used as internal contrast; and (2), extracting chick blood sample genome DNA whose sex is defined, making primer specificity check, then extracting chick embryonic disc cell DNA to be detected, making PCR amplification, and utilizing the amplified DNA fragment size to identify the chick embryonic disc sex.