An analysis method of solid-phase radioactive receptor-ligand combination pertains to the field of biomedical technology. The invention uses 50mmol/L of carbonate buffer solution of pH 9.6 to dissolute a ligand, then the ligand is coated in a detachable 96-hole microplate strip, the receptors which are labeled by the radioactive iodine with different concentrations are added in all the coating holes, the reaction is carried out for 2 hours at 4 DEG C, rinsed by the washing liquid by 3 times, the total cpm value which is measured on a Gamma counter of all the holes is taken to be recorded as cpmt, the counter value of the receptors which are not labeled with 200 times of the concentration of the receptors which are labeled by the radioactive iodine in the corresponding holes is taken asa non-specific combination cpm value to be recorded as cpmb, the cpm value of the radioactive receptor-ligand combination in all the holes is cpmt to cpmb; and the radiochemical purity of the receptors which are labeled by the radioactive iodine is not less than 95 percent. The method of the invention is applied in soluble interleukin 15 receptor Alpha subunit, that is, the analysis of sIL-15R Alpha solid-phase radioactive receptor-ligand combination.