The invention discloses a dielectrophoresis method for proteins of malus plants. The steps of the method are as follows: 1) plant materials are ground in liquid nitrogen and are added with an acetone solution which is pre-cooled at a temperature of 20 DEG C below zero and contains a trichloroacetic acid with the concentration of 10g/100ml and beta-mercaptoethanol with the concentration of 0.07ml/100ml and continuously ground to be the homogenate, and the homogenate stands at a temperature of 20 DEG C below zero for 1 hour and centrifuged by the rotary speed of 12000rpm for 15 minutes, and theprecipitation is kept; 2) the precipitation is added with an acetone solution which contains the beta-mercaptoethanol with the concentration of 0.07ml/100ml and the volume of which is 4 times of the volume of the precipitation, and the mixed solution is evenly mixed and stands at a temperature of 20 DEG C below zero for 1 hour, and centrifuged by the rotary speed of 12000rpm for 15 minutes, and the precipitation is kept; 3) the step 2 is repeated for 6 times, and total proteins are obtained; 4) total proteins obtained from the step 3 undergo the electric focusing electrophoresis, and the sample volume of proteins is of 300ug/170mm adhesive tape, and the polyacrylamide gel electrophoresis is conducted, and the silver dyeing is conducted after the electrophoresis.