The invention relates to the field of medical technology, in particular to a method for separating and purifying individual compounds of tectoridin, wild tectoridin, tectorigenin, wild tectorigenin and sub wild tectorigenin from blackberry lily. The column chromatography, recrystallization, and the like, are mostly adopted at home and abroad to separate and purify effective monomers in the blackberry lily. The methods have the problems of high cost, complex operation, serious sample loss, etc. The invention adopts secondary high-speed countercurrent chromatography to separate the individual compounds in the blackberry lily, and particularly the technology of the secondary high-speed countercurrent chromatography with two sets of different solvent systems is adopted to separate target compounds of tectoridin, wild tectoridin, tectorigenin, wild tectorigenin and sub wild tectorigenin from other interference components with similar polarity, thus obtaining target compounds with high purity. The method is simple in operation, high in separation efficiency and good in product purity, can be continuously sample introduced and is suitable for industrial production.